Dysregulation of cell-to-cell connectivity and stomatal patterning by loss-of-function mutation in Arabidopsis chorus (glucan synthase-like 8)

Jessica M Guseman; Jin Suk Lee; Naomi L Bogenschutz; Kylee M Peterson; Rebecca E Virata; Bo Xie; Masahiro M Kanaoka; Zonglie Hong; Keiko U Torii

doi:10.1242/dev.049197

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Dysregulation of cell-to-cell connectivity and stomatal patterning by loss-of-function mutation in Arabidopsis chorus (glucan synthase-like 8)

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Dysregulation of cell-to-cell connectivity and stomatal patterning by loss-of-function mutation in Arabidopsis chorus (glucan synthase-like 8)

Jessica M Guseman, Jin Suk Lee, Naomi L Bogenschutz, Kylee M Peterson, Rebecca E Virata, Bo Xie, Masahiro M Kanaoka, Zonglie Hong and Keiko U Torii

Development (Cambridge), Vol.137(10), pp.1731-1741

05/2010

DOI: https://doi.org/10.1242/dev.049197

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https://hdl.handle.net/2376/114559

PMID: 20430748

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Abstract

Cell Division - genetics

Arabidopsis Proteins - genetics

Arabidopsis Proteins - physiology

Arabidopsis - enzymology

Seeds

Embryonic Development - genetics

Embryonic Development - physiology

Arabidopsis - embryology

Plant Stomata - embryology

Cell Communication - genetics

Glucosyltransferases - physiology

Arabidopsis - genetics

Mutation, Missense - physiology

Plants, Genetically Modified

Body Patterning - genetics

Cell Lineage - genetics

Glucosyltransferases - genetics

Patterning of stomata, valves on the plant epidermis, requires the orchestrated actions of signaling components and cell-fate determinants. To understand the regulation of stomatal patterning, we performed a genetic screen using a background that partially lacks stomatal signaling receptors. Here, we report the isolation and characterization of chorus (chor), which confers excessive proliferation of stomatal-lineage cells mediated by SPEECHLESS (SPCH). chor breaks redundancy among three ERECTA family genes and strongly enhances stomatal patterning defects caused by loss-of-function in TOO MANY MOUTHS. chor seedlings also exhibit incomplete cytokinesis and growth defects, including disruptions in root tissue patterning and root hair cell morphogenesis. CHOR encodes a putative callose synthase, GLUCAN SYNTHASE-LIKE 8 (GSL8), that is required for callose deposition at the cell plate, cell wall and plasmodesmata. Consistently, symplastic macromolecular diffusion between epidermal cells is significantly increased in chor, and proteins that do not normally move cell-to-cell, including a fluorescent protein-tagged SPCH, diffuse to neighboring cells. Such a phenotype is not a general trait caused by cytokinesis defects. Our findings suggest that the restriction of symplastic movement might be an essential step for the proper segregation of cell-fate determinants during stomatal development.

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Title: Dysregulation of cell-to-cell connectivity and stomatal patterning by loss-of-function mutation in Arabidopsis chorus (glucan synthase-like 8)
Creators: Jessica M Guseman - Department of Biology, University of Washington, Seattle, WA 98195, USA
Jin Suk Lee
Naomi L Bogenschutz
Kylee M Peterson
Rebecca E Virata
Bo Xie
Masahiro M Kanaoka
Zonglie Hong
Keiko U Torii
Publication Details: Development (Cambridge), Vol.137(10), pp.1731-1741
Academic Unit: Center for Reproductive Biology
Publisher: England
Identifiers: 99900548164801842
Language: English
Resource Type: Journal article