EDTA a novel inducer of pisatin, a phytoalexin indicator of the non-host resistance in peas

Lee A Hadwiger; Kiwamu Tanaka

doi:10.3390/molecules20010024

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EDTA a novel inducer of pisatin, a phytoalexin indicator of the non-host resistance in peas

Journal article

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EDTA a novel inducer of pisatin, a phytoalexin indicator of the non-host resistance in peas

Lee A Hadwiger and Kiwamu Tanaka

Molecules (Basel, Switzerland), Vol.20(1), pp.24-34

12/23/2014

DOI: https://doi.org/10.3390/molecules20010024

Handle:

https://hdl.handle.net/2376/106168

PMCID: PMC6272189

PMID: 25546618

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https://doi.org/10.3390/molecules20010024View

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Abstract

Edetic Acid - chemistry

Sesquiterpenes - metabolism

Genes, Plant

Pterocarpans - chemistry

Base Sequence

Peas - microbiology

DNA Damage

DNA Primers

Peas - genetics

Real-Time Polymerase Chain Reaction

Fusarium - pathogenicity

Pea pod endocarp suppresses the growth of an inappropriate fungus or non-pathogen by generating a "non-host resistance response" that completely suppresses growth of the challenging fungus within 6 h. Most of the components of this resistance response including pisatin production can be elicited by an extensive number of both biotic and abiotic inducers. Thus this phytoalexin serves as an indicator to be used in evaluating the chemical properties of inducers that can initiate the resistance response. Many of the pisatin inducers are reported to interact with DNA and potentially cause DNA damage. Here we propose that EDTA (ethylenediaminetetraacetic acid) is an elicitor to evoke non-host resistance in plants. EDTA is manufactured as a chelating agent, however at low concentration it is a strong elicitor, inducing the phytoalexin pisatin, cellular DNA damage and defense-responsive genes. It is capable of activating complete resistance in peas against a pea pathogen. Since there is also an accompanying fragmentation of pea DNA and alteration in the size of pea nuclei, the potential biochemical insult as a metal chelator may not be its primary action. The potential effects of EDTA on the structure of DNA within pea chromatin may assist the transcription of plant defense genes.

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Title: EDTA a novel inducer of pisatin, a phytoalexin indicator of the non-host resistance in peas
Creators: Lee A Hadwiger - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA. chitosan@wsu.edu
Kiwamu Tanaka - Department of Plant Pathology, Washington State University, Pullman, WA 99164-6430, USA. kiwamu.tanaka@wsu.edu
Publication Details: Molecules (Basel, Switzerland), Vol.20(1), pp.24-34
Academic Unit: Plant Pathology, Department of
Publisher: Switzerland
Identifiers: 99900546955601842
Language: English
Resource Type: Journal article