Journal article
EQCM immunoassay for phosphorylated acetylcholinesterase as a biomarker for organophosphate exposures based on selective zirconia adsorption and enzyme-catalytic precipitation
Biosensors & bioelectronics, Vol.24(8), pp.2377-2383
2009
Handle:
https://hdl.handle.net/2376/103009
PMID: 19135350
Abstract
A zirconia (ZrO2) adsorption-based immunoassay by electrochemical quartz crystal microbalance (EQCM) has been initially developed, aiming at the detection of phosphorylated acetylcholinesterase (Phospho-AChE) as a potential biomarker for bio-monitoring exposures to organophosphate (OP) pesticides and chemical warfare agents. Hydroxyl-derivatized monolayer was preferably chosen to modify the crystal serving as the template for directing the electro-deposition of ZrO2 film with uniform nanostructures. The resulting ZrO2 film was utilized to selectively capture Phospho-AChE from the sample media. Horseradish peroxidase (HRP)-labeled anti-AChE antibodies were further employed to recognize the captured phosphorylated proteins. Enzyme-catalytic oxidation of the benzidine substrate resulted in the accumulation of insoluble product on the functionalized crystal. Ultrasensitive EQCM quantification by mass-amplified frequency responses as well as rapid qualification by visual color changes of product could be thus, achieved. Moreover, 4-chloro-1-naphthol (CN) was studied as an ideal chromogenic substrate for the enzyme-catalytic precipitation. Experimental results show that the developed EQCM technique can allow for the detection of Phospho-AChE in human plasma with a detection limit of 0.020 nM. Such an EQCM immunosensing format opens a new door towards the development of simple, sensitive, and field-applicable biosensor for biologically monitoring low-level OP exposures. (C) 2009 Elsevier B.V. All rights reserved.
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Details
- Title
- EQCM immunoassay for phosphorylated acetylcholinesterase as a biomarker for organophosphate exposures based on selective zirconia adsorption and enzyme-catalytic precipitation
- Creators
- HUA WANG - Pacific Norwest National Laboratory, Richland, WA 99352, United StatesJUN WANG - Pacific Norwest National Laboratory, Richland, WA 99352, United StatesDaiwon CHOI - Pacific Norwest National Laboratory, Richland, WA 99352, United StatesZHIWEN TANG - Pacific Norwest National Laboratory, Richland, WA 99352, United StatesHONG WU - Pacific Norwest National Laboratory, Richland, WA 99352, United StatesYuehe Lin - Pacific Norwest National Laboratory, Richland, WA 99352, United States
- Publication Details
- Biosensors & bioelectronics, Vol.24(8), pp.2377-2383
- Academic Unit
- School of Mechanical and Materials Engineering
- Publisher
- Elsevier; Kidlington
- Number of pages
- 7
- Grant note
- NS058161-01 / National Institutes of Health CounterACT Program through the National Institute of Neurological Disorders and Stroke; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) DE-AC05-76RL01830 / DOE; United States Department of Energy (DOE) U01NS058161 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS)
- Identifiers
- 99900546569201842
- Language
- English
- Resource Type
- Journal article