ERK phosphorylation regulates sleep and plasticity in Drosophila

William M Vanderheyden; Jason R Gerstner; Anne Tanenhaus; Jerry C Yin; Paul J Shaw

doi:10.1371/journal.pone.0081554

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ERK phosphorylation regulates sleep and plasticity in Drosophila

Journal article

Open access

ERK phosphorylation regulates sleep and plasticity in Drosophila

William M Vanderheyden, Jason R Gerstner, Anne Tanenhaus, Jerry C Yin and Paul J Shaw

PloS one, Vol.8(11), pp.e81554-e81554

2013

DOI: https://doi.org/10.1371/journal.pone.0081554

Handle:

https://hdl.handle.net/2376/116397

PMCID: PMC3828275

PMID: 24244744

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Abstract

Phosphorylation

Animals

Animals, Genetically Modified

Drosophila - metabolism

Drosophila Proteins - genetics

Extracellular Signal-Regulated MAP Kinases - metabolism

Sleep - physiology

Drosophila Proteins - metabolism

Extracellular Signal-Regulated MAP Kinases - genetics

Drosophila - physiology

Drosophila - enzymology

Given the relationship between sleep and plasticity, we examined the role of Extracellular signal-regulated kinase (ERK) in regulating baseline sleep, and modulating the response to waking experience. Both sleep deprivation and social enrichment increase ERK phosphorylation in wild-type flies. The effects of both sleep deprivation and social enrichment on structural plasticity in the LNvs can be recapitulated by expressing an active version of ERK (UAS-ERK(SEM)) pan-neuronally in the adult fly using GeneSwitch (Gsw) Gsw-elav-GAL4. Conversely, disrupting ERK reduces sleep and prevents both the behavioral and structural plasticity normally induced by social enrichment. Finally, using transgenic flies carrying a cAMP response Element (CRE)-luciferase reporter we show that activating ERK enhances CRE-Luc activity while disrupting ERK reduces it. These data suggest that ERK phosphorylation is an important mediator in transducing waking experience into sleep.

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Title: ERK phosphorylation regulates sleep and plasticity in Drosophila
Creators: William M Vanderheyden - Department of Anesthesiology, University of Michigan Medical School, Ann Arbor, Michigan, United States of America ; Department of Anatomy, Washington University Medical School, Saint Louis, Missouri, United States of America
Jason R Gerstner
Anne Tanenhaus
Jerry C Yin
Paul J Shaw
Publication Details: PloS one, Vol.8(11), pp.e81554-e81554
Academic Unit: Biomedical Sciences, Department of
Publisher: United States
Grant note: 2 R01 NS051305-06 / NINDS NIH HHS R01 NS076980 / NINDS NIH HHS T32 GM007507 / NIGMS NIH HHS P30 HD003352 / NICHD NIH HHS R01 NS051305 / NINDS NIH HHS NS057105 / NINDS NIH HHS P30 NS057105 / NINDS NIH HHS
Identifiers: 99900548000001842
Language: English
Resource Type: Journal article