Journal article
Early Induction of Humoral and Cellular Immune Responses during Experimental Mycobacterium avium subsp. paratuberculosis Infection of Calves
Infection and immunity, Vol.71(9), pp.5130-5138
09/2003
Handle:
https://hdl.handle.net/2376/105234
PMCID: PMC187349
PMID: 12933856
Abstract
Johne's disease (paratuberculosis) of cattle is widespread and causes significant economic losses for producers due to decreased production and poor health of affected animals. The chronic nature of the disease and the lack of a reproducible model of infection hinder research efforts. In the present study, instillation of
Mycobacterium avium
subsp.
paratuberculosis
into the tonsillar crypts of neonatal calves resulted in peripheral colonization as detected by antemortem culture of feces and postmortem (320 days postchallenge) culture of intestinal tissues. Antigen-specific blastogenic, gamma interferon (IFN-γ), and nitric oxide responses by blood mononuclear cells from infected calves exceeded prechallenge responses beginning 194 days postchallenge. Upon in vitro stimulation with paratuberculosis antigens, CD4
+
cells from infected calves proliferated, produced IFN-γ, and increased expression of CD26 and CD45RO (indicative of an activated memory phenotype). Utilizing a lipoarabinomannan-based enzyme-linked immunosorbent assay, specific serum immunoglobulin was detected as early as 134 days postchallenge and generally increased after this time point. Two antigens of ∼50 and ∼60 kDa were particularly immunodominant early in infection, as shown by immunoblot with serum collected within 2 weeks postchallenge. Findings indicate that the intratonsillar inoculation route will prove useful as an experimental model for paratuberculosis infection. Additionally, this study confirms that mycobacteria-specific antibody is detectable early in the course of experimental Johne's disease, even preceding the development of specific cell-mediated responses.
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Details
- Title
- Early Induction of Humoral and Cellular Immune Responses during Experimental Mycobacterium avium subsp. paratuberculosis Infection of Calves
- Creators
- W. R Waters - Bacterial Diseases of Livestock Research UnitJ. M Miller - Bacterial Diseases of Livestock Research UnitM. V Palmer - Bacterial Diseases of Livestock Research UnitJ. R Stabel - Bacterial Diseases of Livestock Research UnitD. E Jones - Bacterial Diseases of Livestock Research UnitK. A Koistinen - Bacterial Diseases of Livestock Research UnitE. M Steadham - Bacterial Diseases of Livestock Research UnitM. J Hamilton - Bacterial Diseases of Livestock Research UnitW. C Davis - Bacterial Diseases of Livestock Research UnitJ. P Bannantine - Bacterial Diseases of Livestock Research Unit
- Publication Details
- Infection and immunity, Vol.71(9), pp.5130-5138
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- American Society for Microbiology
- Identifiers
- 99900546665501842
- Language
- English
- Resource Type
- Journal article