Early development of cytotoxic T lymphocytes in neonatal foals following oral inoculation with Rhodococcus equi

Seth P Harris; Melissa T Hines; Robert H Mealey; Debra C Alperin; Stephen A Hines

doi:10.1016/j.vetimm.2011.03.015

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Early development of cytotoxic T lymphocytes in neonatal foals following oral inoculation with Rhodococcus equi

Journal article

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Early development of cytotoxic T lymphocytes in neonatal foals following oral inoculation with Rhodococcus equi

Seth P Harris, Melissa T Hines, Robert H Mealey, Debra C Alperin and Stephen A Hines

Veterinary immunology and immunopathology, Vol.141(3-4), pp.312-316

06/15/2011

DOI: https://doi.org/10.1016/j.vetimm.2011.03.015

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https://hdl.handle.net/2376/114006

PMCID: PMC3345954

PMID: 21481947

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https://doi.org/10.1016/j.vetimm.2011.03.015View

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Abstract

Animals, Newborn

Animals

Disease Susceptibility

Actinomycetales Infections - immunology

Actinomycetales Infections - microbiology

Aging

Horses

Horse Diseases - microbiology

Rhodococcus equi

Horse Diseases - immunology

T-Lymphocytes, Cytotoxic - physiology

Actinomycetales Infections - veterinary

Rhodococcus equi is an important respiratory pathogen of young foals for which a vaccine has long been sought. Two major impediments to effective vaccination are the functionally immature type I immune responses of neonatal foals and early exposure to the bacterium via the environment. Despite these obstacles, it appears that under specific circumstances foals can develop a protective immune response. In this study we investigated the protective mechanisms behind oral inoculation of foals with virulent R. equi bacteria. Two foals receiving an oral inoculum demonstrated accelerated development of R. equi specific cytotoxic T lymphocytes (CTL) as evidenced by significant lysis of R. equi infected, ELA-A mismatched cells at 3 weeks of age. As in a previous study, CTL were not detected until 5-6 weeks of age in two control foals. At each time point the ability of foal peripheral blood mononuclear cells (PBMC) to produce IFN-γ following stimulation with live R. equi or extracted cell wall lipids was similar to that of an adult horse control and between foals, regardless of treatment. These results provide a potential mechanism of protection which has previously been shown to occur following oral inoculation, and suggest that the early detection of CTL may be a useful marker for induction of protective immunity.

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Title: Early development of cytotoxic T lymphocytes in neonatal foals following oral inoculation with Rhodococcus equi
Creators: Seth P Harris - Department of Veterinary Microbiology and Pathology, Washington State University, College of Veterinary Medicine, PO Box 647040, Pullman, WA 99164-7040, USA
Melissa T Hines
Robert H Mealey
Debra C Alperin
Stephen A Hines
Publication Details: Veterinary immunology and immunopathology, Vol.141(3-4), pp.312-316
Academic Unit: Veterinary Clinical Sciences, Department of; Veterinary Medicine, College of
Publisher: Netherlands
Grant note: T32 AI007025 / NIAID NIH HHS T32 AI007025-24 / NIAID NIH HHS
Identifiers: 99900547655001842
Language: English
Resource Type: Journal article