Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds

Susmita Bose; Solaiman Tarafder; Amit Bandyopadhyay

doi:10.1007/s10439-016-1646-y

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Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds

Journal article

Open access

Peer reviewed

Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds

Susmita Bose, Solaiman Tarafder and Amit Bandyopadhyay

Annals of biomedical engineering, Vol.45(1), pp.261-272

01/2017

DOI: https://doi.org/10.1007/s10439-016-1646-y

Handle:

https://hdl.handle.net/2376/108109

PMCID: PMC5149117

PMID: 27287311

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https://doi.org/10.1007/s10439-016-1646-yView

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Abstract

Tissue Engineering - methods

Bone Substitutes - pharmacology

Rats

Calcium Phosphates - chemistry

Male

Materials Testing

Magnesium - pharmacology

Rats, Sprague-Dawley

Tissue Scaffolds - chemistry

Silicon - pharmacology

Tissue Engineering - instrumentation

Magnesium - chemistry

Animals

Printing, Three-Dimensional

Silicon - chemistry

Bone Substitutes - chemistry

Calcium Phosphates - pharmacology

Neovascularization, Physiologic

Osteogenesis

The functionality or survival of tissue engineering constructs depends on the adequate vascularization through oxygen transport and metabolic waste removal at the core. This study reports the presence of magnesium and silicon in direct three dimensional printed (3DP) tricalcium phosphate (TCP) scaffolds promotes in vivo osteogenesis and angiogenesis when tested in rat distal femoral defect model. Scaffolds with three different interconnected macro pore sizes were fabricated using direct three dimensional printing. In vitro ion release in phosphate buffer for 30 days showed sustained Mg and Si release from these scaffolds. Histolomorphology and histomorphometric analysis from the histology tissue sections revealed a significantly higher bone formation, between 14 and 20% for 4-16 weeks, and blood vessel formation, between 3 and 6% for 4-12 weeks, due to the presence of magnesium and silicon in TCP scaffolds compared to bare TCP scaffolds. The presence of magnesium in these 3DP TCP scaffolds also caused delayed TRAP activity. These results show that magnesium and silicon incorporated 3DP TCP scaffolds with multiscale porosity have huge potential for bone tissue repair and regeneration.

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Title: Effect of Chemistry on Osteogenesis and Angiogenesis Towards Bone Tissue Engineering Using 3D Printed Scaffolds
Creators: Susmita Bose - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164, USA. sbose@wsu.edu
Solaiman Tarafder - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164, USA
Amit Bandyopadhyay - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164, USA
Publication Details: Annals of biomedical engineering, Vol.45(1), pp.261-272
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: United States
Grant note: R01 AR066361 / NIAMS NIH HHS R01 EB007351 / NIBIB NIH HHS
Identifiers: 99900547200801842
Language: English
Resource Type: Journal article