Journal article
Effect of Prolactin on In Vitro Expression of the Bovine Mammary Immunoglobulin G1 Receptor
Journal of dairy science, Vol.80(1), pp.94-100
01/1997
Handle:
https://hdl.handle.net/2376/109885
PMID: 9120100
Abstract
Explants of mammary tissue from cows in late pregnancy were incubated for 72h in serum-free, hormonally defined media to investigate the regulation of the bovine mammary IgG1 receptor. Treatments included incubation in basal medium alone, basal medium plus estradiol-17β, basal medium plus prolactin, or basal medium plus estradiol-17β and prolactin. α-Lactalbumin production was measured by radioimmunoassay in culture supernatants collected at 24, 48, and 72h. Explants were examined immunohistochemically for expression of the IgG1 receptor at 24, 48, and 72h. α-Lactalbumin concentrations increased, and IgG1 receptor expression decreased, by 72h with explants cultured in the medium containing prolactin. Results suggest that, in addition to its positive lactogenic effect, prolactin decreases expression of the bovine mammary IgG1 receptor.
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Details
- Title
- Effect of Prolactin on In Vitro Expression of the Bovine Mammary Immunoglobulin G1 Receptor
- Creators
- G.M Barrington - Washington State University, Pullman 99164T.E Besser - Washington State University, Pullman 99164C.C Gay - Washington State University, Pullman 99164W.C Davis - Washington State University, Pullman 99164J.J Reeves - Washington State University, Pullman 99164T.B McFadden - Ruakura Agricultural Centre, Hamilton, New Zealand
- Publication Details
- Journal of dairy science, Vol.80(1), pp.94-100
- Academic Unit
- Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547129601842
- Language
- English
- Resource Type
- Journal article