Journal article
Effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (Venerupis philippinarum) and mussel (Mytilus edulis)
International journal of food microbiology, Vol.231, pp.48-53
08/16/2016
Handle:
https://hdl.handle.net/2376/104150
PMID: 27208583
Abstract
The effect of acidic electrolyzed water (AEW) on inactivating Escherichia coli O104:H4, Listeria monocytogenes, Aeromonas hydrophila, Vibrio parahaemolyticus and Campylobacter jejuni in laboratory contaminated live clam (Venerupis philippinarum) and mussel (Mytilus edulis) was investigated. The initial levels of bacterial contamination were: in clam 4.9 to 5.7log10CFU/g, and in mussel 5.1 to 5.5log10CFU/g. Two types of AEW were used for treatment time intervals of 1 and 2h: strong (SAEW) with an available chlorine concentration (ACC) of 20mg/L, pH=3.1, and an oxidation-reduction potential (ORP) of 1150mV, and weak (WAEW) at ACC of 10mg/L, pH=3.55 and ORP of 950mV. SAEW and WAEW exhibited significant inhibitory activity against inoculated bacteria in both shellfish species with significant differences compared to saline solutions treatments (1–2% NaCl) and untreated controls (0h). SAEW showed the largest inhibitory activity, the extent of reduction (log10CFU/g) ranged from 1.4–1.7 for E. coli O104:H4; 1.0–1.6 for L. monocytogenes; 1.3–1.6 for A. hydrophila; 1.0–1.5 for V. parahaemolyticus; and 1.5–2.2 for C. jejuni in both types of shellfish. In comparison, significantly (P<0.05) lower inhibitory effect of WAEW was achieved compared to SAEW, where the extent of reduction (log10CFU/g) ranged from 0.7–1.1 for E. coli O104:H4; 0.6–0.9 for L. monocytogenes; 0.6–1.3 for A. hydrophila; 0.7–1.3 for V. parahaemolyticus; and 0.8–1.9 for C. jejuni in both types of shellfish. Among all bacterial strains examined in this study, AEW induced less bacterial injury (~0.1–1.0log10CFU/g) and more inactivation effect. This study revealed that AEW (10–20mg/L ACC) could be used to reduce bacterial contamination in live clam and mussel, which may help control possible unhygienic practices during production and processing of shellfish without apparent changes in the quality of the shellfish.
•Depuration with AEW reduces bacterial contamination in live clam and mussel.•Depuration with 1–2% NaCl may not cause a reduction in bacterial contamination.•AEW may help control possible unhygienic practices during processing of shellfish.
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Details
- Title
- Effect of acidic electrolyzed water-induced bacterial inhibition and injury in live clam (Venerupis philippinarum) and mussel (Mytilus edulis)
- Creators
- Hamzah M Al-Qadiri - Department of Nutrition and Food Technology, Faculty of Agriculture, The University of Jordan, Amman 11942, JordanMurad A Al-Holy - Department of Clinical Nutrition and Dietetics, Faculty of Allied Health Sciences, Hashemite University, P.O Box 150459, Zarqa 13115, JordanSetareh Ghorban Shiroodi - School of Food Science, Washington State University, Pullman, WA 99164, USAMahmoudreza Ovissipour - School of Food Science, Washington State University, Pullman, WA 99164, USAByju N Govindan - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USANivin Al-Alami - Water, Energy and Environment Center, The University of Jordan, Amman 11942, JordanShyam S Sablani - Department of Biological Systems Engineering, Washington State University, Pullman, WA 99164, USABarbara Rasco - School of Food Science, Washington State University, Pullman, WA 99164, USA
- Publication Details
- International journal of food microbiology, Vol.231, pp.48-53
- Academic Unit
- Biological Systems Engineering, Department of; Food Science, School of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546653101842
- Language
- English
- Resource Type
- Journal article