Journal article
Effect of dialysis or centrifugation on post-thaw motility and fertility of Santa Gertrudis bull semen collected by electroejaculation
Theriogenology, Vol.33(3), pp.733-739
1990
Handle:
https://hdl.handle.net/2376/113773
PMID: 16726769
Abstract
Electroejaculated semen from Santa Gertrudis bulls was used to study the effect of centrifugation (600 x g for 5 min) or dialysis [molecular weight cutoff <14,000 Daltons (Da)] on post-thaw motility and on fertility in beef cattle. Analysis of post-thaw motility showed that the main effects (bulls and semen treatment) were significant (P<0.05). Dialysis significantly improved post-thaw motility in four of seven bulls. Initial volume of seminal plasma in the ejaculate was negatively correlated to post-thaw motility (r = −0.73). No significant improvement in post-thaw motility was observed for bulls with high volumes of ejaculate. In a fertility trial, calving rates of heifers synchronized with PGF
2α and inseminated at 72 and 96 h after the second PGF
2α injection with dialyzed or commercial semen were not statistically different (P>0.05; 54.4% vs 55.4%). These results show that dialysis could be used to improve post-thaw motility of electroejaculated bull semen without altering its fertilizing capacity. However, a high initial volume of seminal plasma seems to have a deleterious effect on sperm freezability that cannot be reversed by dialysis.
Metrics
8 Record Views
Details
- Title
- Effect of dialysis or centrifugation on post-thaw motility and fertility of Santa Gertrudis bull semen collected by electroejaculation
- Creators
- A Tibary - Department of Reproduction, Institute of Agronomy and Veterinary Medicine, Hassan II, Rabat-Instituts BP 6202, MoroccoE.F Graham - Department of Animal Science, University of Minnesota St. Paul, MN 55108 USAA Asri - Societe National de Development de l'Elevage Tour Hassan, Rabat, MoroccoR Boukhliq - Department of Reproduction, Institute of Agronomy and Veterinary Medicine, Hassan II, Rabat-Instituts BP 6202, MoroccoR Deyo - Department of Animal Science, University of Minnesota St. Paul, MN 55108 USA
- Publication Details
- Theriogenology, Vol.33(3), pp.733-739
- Academic Unit
- Veterinary Clinical Sciences, Department of
- Publisher
- Elsevier Inc
- Identifiers
- 99900547452101842
- Language
- English
- Resource Type
- Journal article