Effect of highly branched hyphal morphology on the enhanced production of cellulase in Trichoderma reesei DES-15

Ronglin He; Chen Li; Lijuan Ma; Dongyuan Zhang; Shulin Chen

doi:10.1007/s13205-016-0516-5

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Effect of highly branched hyphal morphology on the enhanced production of cellulase in Trichoderma reesei DES-15

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Effect of highly branched hyphal morphology on the enhanced production of cellulase in Trichoderma reesei DES-15

Ronglin He, Chen Li, Lijuan Ma, Dongyuan Zhang and Shulin Chen

3 Biotech, Vol.6(2), p.214

12/2016

DOI: https://doi.org/10.1007/s13205-016-0516-5

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https://hdl.handle.net/2376/116859

PMCID: PMC5052222

PMID: 28330286

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Abstract

Branching

Cellulase production

Trichoderma reesei

Submerged cultures

Hyphal morphology

The morphology of Trichoderma reesei is a vitally important factor for cellulase productivity. This study investigated the effect of hyphal morphology on cellulase production in the hyper-cellulolytic mutant, T. reesei DES-15. With a distinct morphology, T. reesei DES-15 was obtained through Diethyl sulfite (DES) mutagenesis. The hyphal morphology of DES-15 batch-cultured in a 5-L fermentor was significantly shorter and more branched than the parental strain RUT C30. The cellulase production of DES-15 during batch fermentation was 66 % greater than that of RUT C30 when cultured the same conditions. DES-15 secreted nearly 50 % more protein than RUT C30. The gene expression level of a set of genes (cla4, spa2, ras2, ras1, rhoA, cdc42, and racA) known to be involved in hyphae growth and hyphal branching was measured by quantitative real-time PCR. The transcriptional analysis of these genes demonstrated that a decrease in gene expressions might contribute to the increased hyphal branching seen in DES-15. These results indicated that the highly branching hyphae in DES-15 resulted in increased cellulase production, suggesting that DES-15 may be a good candidate for use in the large-scale production of cellulase.

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Title: Effect of highly branched hyphal morphology on the enhanced production of cellulase in Trichoderma reesei DES-15
Creators: Ronglin He - Tianjin Key Lab of Industrial Microbiology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China
Chen Li - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China
Lijuan Ma - College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People's Republic of China
Dongyuan Zhang - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China. zhang_dy@tib.cas.cn
Shulin Chen - Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, People's Republic of China
Publication Details: 3 Biotech, Vol.6(2), p.214
Academic Unit: Biological Systems Engineering, Department of
Identifiers: 99900582336201842
Language: English
Resource Type: Journal article