Journal article
Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar)
Veterinary immunology and immunopathology, Vol.151(1-2), pp.113-123
01/15/2013
Handle:
https://hdl.handle.net/2376/113992
PMID: 23206403
Abstract
This study aimed to assess systemic and mucosal immune responses of Atlantic salmon (Salmo salar) exposed to two protein-hapten antigens – dinitrophenol (DNP) and fluorescein isothiocyanate (FITC) each conjugated with keyhole limpet haemocyanin (KLH) – administered using different delivery strategies. Fish were exposed to the antigens through different routes, and were given a booster 4 weeks post initial exposure. Both systemic and mucosal antibody responses were measured for a period of 12 weeks using an enzyme-linked immunosorbent assay (ELISA). Only fish exposed to both antigens via intraperitoneal (IP) injection showed increased systemic antibody response starting 6 weeks post immunization. No treatment was able to produce a mucosal antibody response; however there was an increase in antibody levels in the tissue supernatant from skin explants obtained 12 weeks post immunization from fish injected with FITC. Western blots probed with serum and culture supernatant from skin explants showed a specific response against the antigens. In conclusion, IP injection of hapten-antigen in Atlantic salmon was the best delivery route for inducing an antibody response against these antigens in this species. Even though IP injection did not induce an increase in antibody levels in the skin mucus, there was an increased systemic antibody response and an apparent increase of antibody production in mucosal tissues as demonstrated by the increased level of specific antibody levels in supernatants from the tissue explants.
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Details
- Title
- Effect of immunization route on mucosal and systemic immune response in Atlantic salmon (Salmo salar)
- Creators
- Victoria A Valdenegro-Vega - NCMCRS, Locked Bag 1370, University of Tasmania, Launceston TAS 7250, AustraliaPhilip Crosbie - NCMCRS, Locked Bag 1370, University of Tasmania, Launceston TAS 7250, AustraliaBenita Vincent - NCMCRS, Locked Bag 1370, University of Tasmania, Launceston TAS 7250, AustraliaKenneth D Cain - NCMCRS, Locked Bag 1370, University of Tasmania, Launceston TAS 7250, AustraliaBarbara F Nowak - NCMCRS, Locked Bag 1370, University of Tasmania, Launceston TAS 7250, Australia
- Publication Details
- Veterinary immunology and immunopathology, Vol.151(1-2), pp.113-123
- Academic Unit
- Center for Reproductive Biology
- Publisher
- Elsevier B.V
- Identifiers
- 99900547703101842
- Language
- English
- Resource Type
- Journal article