Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1

Yong Zhang; Qingsong Min; Jian Xu; Ke Zhang; Shulin Chen; Haijun Wang; Demao Li

doi:10.1016/j.ejbt.2015.11.006

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Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1

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Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1

Yong Zhang, Qingsong Min, Jian Xu, Ke Zhang, Shulin Chen, Haijun Wang and Demao Li

Electronic Journal of Biotechnology, Vol.19(1), pp.56-60

01/2016

DOI: https://doi.org/10.1016/j.ejbt.2015.11.006

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https://hdl.handle.net/2376/117848

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Abstract

NADPH

Malate

Schizochytrium sp

DHA

Malate involves in the citrate/malate and transhydrogenase cycles to provide precursors for docosahexaenoic acid (DHA) synthesis. The optimal strategy was investigated for increasing DHA production in Schizochytrium species during fermentation. DHA production increased by 47% and reached 5.51g/L when 4g malate/L was added during the rapid lipid accumulation stage in shake-flasks culture. Inducing effects of malate was further investigated through the analysis of three kinetic parameters, including specific cell growth rate (μ), specific glucose consumption rate (qGlu) and DHA formation rate (qDHA). DHA concentration was enhanced through a novel fed-batch strategy to a maximum value of 30.7g/L, giving a yield of 0.103g DHA/g glucose and a productivity of 284mgL-1h-1. A novel malate feeding strategy was developed that enhanced DHA yield and productivity of Schizochytrium species which may offer a desirable method for industrial applications.

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Title: Effect of malate on docosahexaenoic acid production from Schizochytrium sp. B4D1
Creators: Yong Zhang - College of Animal and Veterinary Science, Shenyang Agriculture University, Shenyang, 110866, China
Qingsong Min - College of Animal and Veterinary Science, Shenyang Agriculture University, Shenyang, 110866, China
Jian Xu - College of Animal and Veterinary Science, Shenyang Agriculture University, Shenyang, 110866, China
Ke Zhang - Tianjin Key Laboratory for Industrial Biosystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, PR China
Shulin Chen - Tianjin Key Laboratory for Industrial Biosystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, PR China
Haijun Wang - Tianjin Key Laboratory for Industrial Biosystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, PR China
Demao Li - Tianjin Key Laboratory for Industrial Biosystems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, PR China
Publication Details: Electronic Journal of Biotechnology, Vol.19(1), pp.56-60
Publisher: Elsevier B.V
Identifiers: 99900582327301842
Language: English
Resource Type: Journal article