Journal article
Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis
Journal of biotechnology, Vol.168(4), pp.470-477
12/2013
Handle:
https://hdl.handle.net/2376/109564
PMID: 24129314
Abstract
A low-cost of cellulase achieved through improving fermentation technology remains a key requirement for commercialization of cellulosic biofuels and biochemicals. pH plays a very important role in the process of cellulase synthesis by Trichoderma reesei. In this work, effects of pH on the production and production rates of three cellulase components (endoglucanase, exoglucanase, β-glucosidase) and mycelial morphology were studied. Production rates of the cellulase components were kept highest and the mycelial morphology was maintained at the optimal status by developing a phased pH control strategy in order to improve cellulase production. Cellulase production in terms of filter paper activity and β-glucosidase production in batch fermentation increased 17.6% and 22%. Saccharification efficiency of the enzyme obtained by pH control was evaluated by hydrolyzing pretreated corn cob. Saccharification yield increased significantly (up to 26.2%) compared with that without pH control. These results add new knowledge on approach for improving cellulase production.
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Details
- Title
- Effect of pH on cellulase production and morphology of Trichoderma reesei and the application in cellulosic material hydrolysis
- Creators
- Chen Li - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, ChinaZhenhua YangRonglin He Can ZhangDongyuan ZhangShulin ChenLijuan Ma
- Publication Details
- Journal of biotechnology, Vol.168(4), pp.470-477
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Netherlands
- Identifiers
- 99900547347801842
- Language
- English
- Resource Type
- Journal article