Journal article
Effect of parental exposure to trenbolone and the brominated flame retardant BDE-47 on fertility in rainbow trout ( Oncorhynchus mykiss)
Marine environmental research, Vol.66(1), pp.47-49
2008
Handle:
https://hdl.handle.net/2376/116788
PMCID: PMC2441647
PMID: 18397801
Abstract
We exposed sexually maturing male rainbow trout (
Oncorhynchus mykiss) to BDE-47 (a polybrominated diphenyl ether) and female rainbow trout to trenbolone (an anabolic steroid). Male trout were orally exposed for 17 days to 55
μg/kg/day BDE-47 and female trout continuously exposed for 60–77 days to a measured trenbolone water concentration of 35
ng/L. After the exposure, eggs and semen were collected and in vitro fertilization trials performed using a sperm:egg ratio of 300,000:1. In the BDE-47 study, eggs from control females were fertilized with semen from exposed males, while in the trenbolone study, eggs from exposed females were fertilized with semen from control males. All treatments were evaluated at two–three early developmental time-points representing first cleavage (0.5
day), embryonic keel (9
days), and eyed stages (19
days), respectively. The results indicated that BDE-47 exposure did not alter fertility as embryonic survival was similar between control and exposed groups. Trenbolone exposure also did not alter embryo survival. However, in the embryos fertilized with eggs from trenbolone exposed females, a noticeable delay in developmental progress was observed. On day 19 when eye development is normally complete, the majority of the embryos either lacked eyes or displayed under-developed eyes, in contrast to control embryos. This finding suggests steroidal androgen exposure in sexually maturing female rainbow trout can impact developmental timing of F1 offspring.
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Details
- Title
- Effect of parental exposure to trenbolone and the brominated flame retardant BDE-47 on fertility in rainbow trout ( Oncorhynchus mykiss)
- Creators
- Irv Schultz - Battelle, Pacific Northwest Division – Marine Sciences Laboratory, 1529 W Sequim Bay Road, Sequim, WA 98382, United StatesKim H Brown - University of Idaho, Department of Biological Sciences and Center for Reproductive Biology, Moscow, ID, United StatesJames J Nagler - University of Idaho, Department of Biological Sciences and Center for Reproductive Biology, Moscow, ID, United States
- Publication Details
- Marine environmental research, Vol.66(1), pp.47-49
- Academic Unit
- UNKNOWN
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547761001842
- Language
- English
- Resource Type
- Journal article