Journal article
Effect of target cell availability on HIV-1 production in vitro
AIDS (London), Vol.16(3), pp.341-345
02/15/2002
Handle:
https://hdl.handle.net/2376/106629
PMID: 11834944
Abstract
The recovery of CD4 target cells following antiretroviral therapy may facilitate virus production and escape from antiretroviral suppression. To address this hypothesis, we directly examined whether the CD4 target cell number increases viral production in the presence of suboptimal therapy.
The effect of the CD4 T cell number on HIV-1 replication with a suboptimal dose of zidovudine was studied in vitro.
Varying numbers of CD4 T cells were infected with HIV-1 and treated with 1 nM zidovudine. Virus production was measured by p24 antigen capture enzyme-linked immunosorbent assay. Partial sequencing of HIV-1 pol was performed to assess zidovudine-resistant mutations.
Wild type virus production was found to increase eightfold in cultures with 100 x 10(4) cells compared with cultures with 10 x 10(4) cells. The IC90 of zidovudine was 4 logs higher in cultures with 16 x 10(4) cells compared with cultures with 1 x 10(4) cells. No zidovudine-resistant mutations were found.
Target cell availability may play a direct role in wild type HIV-1 resurgence following therapy.
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Details
- Title
- Effect of target cell availability on HIV-1 production in vitro
- Creators
- Elissa J Schwartz - Division of Nephrology, Mount Sinai Medical Center, One Gustave L. Levy Place, New York, NY 10029, USA. elissa.schwartz@mssm.eduAvidan U NeumannAvelino V TeixeiraLeslie A BruggemanJay RappaportAlan S PerelsonPaul E Klotman
- Publication Details
- AIDS (London), Vol.16(3), pp.341-345
- Academic Unit
- Biological Sciences, School of
- Publisher
- England
- Grant note
- RR 06555 / NCRR NIH HHS DK 50795 / NIDDK NIH HHS
- Identifiers
- 99900547070501842
- Language
- English
- Resource Type
- Journal article