Journal article
Effects of Ara‐C on neutral sphingomyelinase and mitogen‐and stress‐activated protein kinases in T‐lymphocyte cell lines
IUBMB life, Vol.40(4), pp.709-719
11/1996
Handle:
https://hdl.handle.net/2376/116445
PMID: 8950029
Abstract
Neutral sphingomyelinase (SMase)1 can be activated by extracellular signals to produce ceramide, which may affect mitogen‐activated protein kinase (MAPK) activities. Neutral SMase activity was assessed in membranes from Jurkat, a human T‐cell line, and EL4, a murine T‐cell line. Ara‐C activated SMase within 10 minutes in both Jurkat and EL4 cells, while phorbol ester (PMA) had no effect. PMA, but not Ara‐C or ceramides, activated ERK MAPKs in Jurkat and EL4. PMA acted synergistically with ionomycin to activate JNK MAPKs in Jurkat and EL4 within 10 minutes. Ara‐C activated JNKs only after prolonged incubation (90‐120 minutes). Thus, ceramide is not a positive signal for ERK activation in T‐cell lines. The effects of Ara‐C on JNK activity may be mediated through secondary response pathways.
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Details
- Title
- Effects of Ara‐C on neutral sphingomyelinase and mitogen‐and stress‐activated protein kinases in T‐lymphocyte cell lines
- Creators
- Cynthia D BradshawKrishna M EllaAydrian L ThomasChen QiKathryn E Meier
- Publication Details
- IUBMB life, Vol.40(4), pp.709-719
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Informa Healthcare; UK
- Number of pages
- 11
- Identifiers
- 99900547306601842
- Language
- English
- Resource Type
- Journal article