Effects of Iron on Physical and Mechanical Properties, and Osteoblast Cell Interaction in β-Tricalcium Phosphate

Sahar Vahabzadeh; Susmita Bose

doi:10.1007/s10439-016-1724-1

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Effects of Iron on Physical and Mechanical Properties, and Osteoblast Cell Interaction in β-Tricalcium Phosphate

Journal article

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Effects of Iron on Physical and Mechanical Properties, and Osteoblast Cell Interaction in β-Tricalcium Phosphate

Sahar Vahabzadeh and Susmita Bose

Annals of biomedical engineering, Vol.45(3), pp.819-828

03/2017

DOI: https://doi.org/10.1007/s10439-016-1724-1

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https://hdl.handle.net/2376/115187

PMCID: PMC5608263

PMID: 27896489

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Abstract

Cell Line

Cell Proliferation

Ceramics - chemistry

Humans

Iron - chemistry

Surface Properties

Cell Communication

Calcium Phosphates - chemistry

Osteoblasts - metabolism

Iron (Fe) is a vital element and its deficiency causes abnormal bone metabolism. We investigated the effects of Fe and its concentration in β-tricalcium phosphate (β-TCP) on physicomechanical properties and in vitro proliferation and differentiation of osteoblasts. Our results showed that Fe addition at concentrations of 0.5 wt.% (0.5 Fe-TCP) and 1.0 wt.% (1.0 Fe-TCP) inhibits the β-TCP to α-TCP phase transformation at sintering temperature of 1250 °C. Addition of 0.25 wt.% Fe (0.25 Fe-TCP) increased the compressive strength of β-TCP from 167.27 ± 16.2 to 227.10 ± 19.3 MPa. After 3 days of culture, surfaces of 0.5 Fe-TCP and 1.0 Fe-TCP samples were covered by osteoblast cells, compared to that of pure and 0.25 Fe-TCP. Cells grew to confluency on all Fe-doped samples after 7 days of culture and monolayer sheet-like cellular structure was found at 11 days. Optical cell density and alkaline phosphatase activity were significantly higher on Fe-doped samples and the highest values were found in 0.5 Fe-TCP samples. Our results show that Fe concentration had significant effect on physical and mechanical properties of TCP ceramics, and also on the in vitro osteoblast cellular interactions in TCP ceramics.

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Title: Effects of Iron on Physical and Mechanical Properties, and Osteoblast Cell Interaction in β-Tricalcium Phosphate
Creators: Sahar Vahabzadeh - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164-2920, USA
Susmita Bose - W. M. Keck Biomedical Materials Research Laboratory, School of Mechanical and Materials Engineering, Washington State University, Pullman, WA, 99164-2920, USA. sbose@wsu.edu
Publication Details: Annals of biomedical engineering, Vol.45(3), pp.819-828
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: United States
Grant note: R01 AR066361 / NIAMS NIH HHS
Identifiers: 99900547551201842
Language: English
Resource Type: Journal article