Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal

Buom-Yong Ryu; Kyle E Orwig; Jon M Oatley; Mary R Avarbock; Ralph L Brinster

doi:10.1634/stemcells.2005-0580

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Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal

Journal article

Open access

Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal

Buom-Yong Ryu, Kyle E Orwig, Jon M Oatley, Mary R Avarbock and Ralph L Brinster

Stem cells (Dayton, Ohio), Vol.24(6), pp.1505-1511

06/2006

DOI: https://doi.org/10.1634/stemcells.2005-0580

Handle:

https://hdl.handle.net/2376/110361

PMCID: PMC5501308

PMID: 16456131

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Abstract

Gene Expression

Testis - metabolism

Testis - cytology

Spermatogenesis

Mice, Inbred C57BL

Glial Cell Line-Derived Neurotrophic Factor - genetics

Infertility, Male - etiology

Male

Mice, Transgenic

Stem Cells - cytology

Infertility, Male - pathology

Stem Cells - metabolism

Glial Cell Line-Derived Neurotrophic Factor Receptors - genetics

Spermatogonia - metabolism

Aging - pathology

Stem Cell Transplantation

Animals

Aging - genetics

Cell Differentiation

Mice

Spermatogonia - transplantation

Spermatogonia - cytology

Aging is evident in most tissues and organ systems, but the mechanisms of aging are difficult to identify and poorly understood. Here, we test the hypothesis that aging results in uncorrected defects in stem cell and/or niche function, which lead to system failure. We used the spermatogonial stem cell (SSC) transplantation assay to determine the effect of aging on testis stem cell/niche function in mice. Between 12 and 24 months of age, male mice experienced a declining level of fertility associated with decreased testis weight, level of spermatogenesis, and total stem cell content. However, when stem cells were consecutively passaged at 3-month intervals to testes of young males, these stem cells continued to produce spermatogenesis for more than 3 years. Thus, SSC self-renewal continues long past the normal life span of the animal when the stem cell is continually maintained in a young niche/microenvironment. Moreover, these data suggest that infertility in old males results from deterioration of the SSC niche and failure to support an appropriate balance between stem cell self-renewal and differentiation.

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Title: Effects of aging and niche microenvironment on spermatogonial stem cell self-renewal
Creators: Buom-Yong Ryu - Department of Animal Biology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, 19104, USA
Kyle E Orwig
Jon M Oatley
Mary R Avarbock
Ralph L Brinster
Publication Details: Stem cells (Dayton, Ohio), Vol.24(6), pp.1505-1511
Academic Unit: Molecular Biosciences, School of
Publisher: United States
Grant note: AG 024992 / NIA NIH HHS R01 HD044445 / NICHD NIH HHS HD 044445 / NICHD NIH HHS
Identifiers: 99900547118001842
Language: English
Resource Type: Journal article