Journal article
Effects of ampholyte dissociation constants on protein separation in on-chip isoelectric focusing
Journal of nanoscience and nanotechnology, Vol.8(7), pp.3719-3728
07/2008
Handle:
https://hdl.handle.net/2376/108594
PMID: 19051929
Abstract
Numerical simulations are presented for ampholyte-based isoelectric focusing in 2D microgeometries. In this study, model proteins are focused in the presence of 25 biprotic ampholytes under an applied electric field. Each protein is considered as a simple polypeptide having ten charge states, while the biprotic ampholytes are selected to generate a shallow pH range of 6 to 9. Straight and contraction-expansion microchannels are considered here, and a nominal electric field of 300 V/cm is maintained for separation of proteins. Six distinct values of deltapKs between 1 and 3.5 are investigated for ampholytes to form pH profiles in a 1 cm long microchannel. Simulation results show that relatively larger values of deltapK(deltapK > 3) are required to form stepless pH profiles in the system. The peak heights and differential resolutions of focused proteins are much higher for lower values of deltapK for which a stepped pH profile is evident. For each protein, the time it takes for the two edges of a peak to merge increases linearly with deltapK, while the focusing time goes up exponentially with increasing deltapK. Both merging and focusing times of protein are higher for contraction-expansion microchannel than those of straight microchannel. For a particular value of deltapK, the contracted "Zoom" region of contraction-expansion channel is able to form more tightly focused bands than the expanded region.
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Details
- Title
- Effects of ampholyte dissociation constants on protein separation in on-chip isoelectric focusing
- Creators
- Jaesool Shim - School of Mechanical and Materials Engineering, Washington State University, Pullman, WA 99164-2920, USAPrashanta DuttaCornelius F Ivory
- Publication Details
- Journal of nanoscience and nanotechnology, Vol.8(7), pp.3719-3728
- Academic Unit
- Chemical Engineering and Bioengineering, School of; Mechanical and Materials Engineering, School of
- Publisher
- United States
- Identifiers
- 99900546823201842
- Language
- English
- Resource Type
- Journal article