Journal article
Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies
Bioresource technology, Vol.102(24), pp.11115-11120
2011
Handle:
https://hdl.handle.net/2376/115687
PMID: 21507624
Abstract
The objective of this work is to investigate the effects of cellulase loading and β-glucosidase supplementation on enzymatic hydrolysis of pretreated Dacotah switchgrass. To assess the difference among various pretreatment methods, the profiles of sugars and intermediates were determined for differently treated substrates. For all pretreatments, 72
h glucan/xylan digestibilities increased sharply with enzyme loading up to 25
mg protein/g-glucan, after which the response varied depending on the pretreatment method. For a fixed level of enzyme loading, dilute sulfuric acid (DA), SO
2, and Lime pretreatments exhibited higher digestibility than the soaking in aqueous ammonia (SAA) and ammonia fiber expansion (AFEX). Supplementation of Novozyme-188 to Spezyme-CP improved the 72
h glucan digestibility only for the SAA treated samples. The effect of β-glucosidase supplementation was discernible only at the early phase of hydrolysis where accumulation of cellobiose and oligomers is significant. Addition of β-glucosidase increased the xylan digestibility of alkaline treated samples due to the β-xylosidase activity present in Novozyme-188.
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Details
- Title
- Effects of enzyme loading and β-glucosidase supplementation on enzymatic hydrolysis of switchgrass processed by leading pretreatment technologies
- Creators
- Venkata Ramesh Pallapolu - Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, USAY.Y Lee - Department of Chemical Engineering, Auburn University, 212 Ross Hall, Auburn, AL 36849, USARebecca J Garlock - Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USAVenkatesh Balan - Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USABruce E Dale - Department of Chemical Engineering and Materials Science, Michigan State University, 3900 Collins Road, Lansing, MI 48910, USAYoungmi Kim - LORRE, Department of Agricultural and Biological Engineering, Purdue University, 500 Central Dr., West Lafayette, IN 47907, USANathan S Mosier - LORRE, Department of Agricultural and Biological Engineering, Purdue University, 500 Central Dr., West Lafayette, IN 47907, USAMichael R Ladisch - LORRE, Department of Agricultural and Biological Engineering, Purdue University, 500 Central Dr., West Lafayette, IN 47907, USAMatthew Falls - Department of Chemical Engineering, Texas A&M University, 3122 TAMU, College Station, TX 77843-3122, USAMark T Holtzapple - Department of Chemical Engineering, Texas A&M University, 3122 TAMU, College Station, TX 77843-3122, USARocio Sierra-Ramirez - Universidad de los Andes Chemical Engineering Department Grupo de Conversion de Energia, Bogotá, ColombiaJian Shi - Center for Environmental Research and Technology, Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California at Riverside, 1084 Columbia Avenue, Riverside, CA 92507, USAMirvat A Ebrik - Center for Environmental Research and Technology, Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California at Riverside, 1084 Columbia Avenue, Riverside, CA 92507, USATim Redmond - Center for Environmental Research and Technology, Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California at Riverside, 1084 Columbia Avenue, Riverside, CA 92507, USABin Yang - Center for Environmental Research and Technology, Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California at Riverside, 1084 Columbia Avenue, Riverside, CA 92507, USACharles E Wyman - Center for Environmental Research and Technology, Department of Chemical and Environmental Engineering, Bourns College of Engineering, University of California at Riverside, 1084 Columbia Avenue, Riverside, CA 92507, USABryon S Donohoe - Chemical and Biosciences Center, National Renewable Energy Laboratory, 1617 Cole Blvd., Golden, CO 80401, USATodd B Vinzant - Chemical and Biosciences Center, National Renewable Energy Laboratory, 1617 Cole Blvd., Golden, CO 80401, USARichard T Elander - Chemical and Biosciences Center, National Renewable Energy Laboratory, 1617 Cole Blvd., Golden, CO 80401, USABonnie Hames - Ceres, Inc., 1535 Rancho Conejo Blvd., Thousand Oaks, CA 91320, USASteve Thomas - Ceres, Inc., 1535 Rancho Conejo Blvd., Thousand Oaks, CA 91320, USARyan E Warner - Genencor, A Danisco Division, 925 Page Mill Road, Palo Alto, CA 94304, USA
- Publication Details
- Bioresource technology, Vol.102(24), pp.11115-11120
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547555301842
- Language
- English
- Resource Type
- Journal article