Effects of ethanol on embryonic and neonatal rat testes in organ cultures

Hui Li; Kwan Hee Kim

doi:10.1002/j.1939-4640.2003.tb02723.x

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Effects of ethanol on embryonic and neonatal rat testes in organ cultures

Journal article

Peer reviewed

Effects of ethanol on embryonic and neonatal rat testes in organ cultures

Hui Li and Kwan Hee Kim

Journal of andrology, Vol.24(5), pp.653-660

09/2003

DOI: https://doi.org/10.1002/j.1939-4640.2003.tb02723.x

Handle:

https://hdl.handle.net/2376/114750

PMID: 12954654

Abstract

Apoptosis - drug effects

Glycoproteins - metabolism

Male

Sertoli Cells - drug effects

Testis - drug effects

Central Nervous System Depressants - pharmacology

DNA-Binding Proteins - metabolism

Spermatocytes - drug effects

Testis - embryology

Sertoli Cells - cytology

Testicular Hormones - metabolism

Anti-Mullerian Hormone

Female

Fetus

Organ Culture Techniques

Animals, Newborn

Sertoli Cells - metabolism

GATA4 Transcription Factor

Testis - cytology

Rats

Receptors, Retinoic Acid - metabolism

Rats, Sprague-Dawley

Cell Division - drug effects

Pregnancy

Transcription Factors - metabolism

Ethanol - pharmacology

Animals

Spermatocytes - cytology

Retinoic Acid Receptor alpha

Ethanol exposure in adult animals and humans has shown to elicit significant inhibitory effects on the function of male reproduction, but consequences of ethanol exposure on the embryonic and early postnatal testis development are not known. The current study investigated the effect of ethanol on embryonic and neonatal testis development using an organ culture technique. In embryonic day 13 (E13) testis organ cultures, ethanol had no effect on the testicular cord formation, the expression of Müllerian-inhibiting substance (MIS) in Sertoli cells or the number of gonocytes. Similarly, in the ethanol-treated embryonic day 18 (E18) testes, both the number of gonocytes and the expression of GATA-4 and MIS were similar to those from the control testes. In contrast, in postnatal day 3 (P3) testes, ethanol at concentrations of 150 and 200 mM significantly decreased the number of gonocytes without affecting the expression of GATA-4 and MIS in Sertoli cells. This effect was shown to be resulting from the enhanced apoptosis of gonocytes. In addition, ethanol abnormally activated retinoic acid receptor alpha (RARalpha), as indicated by increased nuclear localization of RARalpha with increasing doses of ethanol treatment. These observations suggest that the effect of ethanol on testis varies at different stages during embryonic and neonatal testis development. Furthermore, germ cells may be the main target for the action of ethanol on the early postnatal testis.

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Title: Effects of ethanol on embryonic and neonatal rat testes in organ cultures
Creators: Hui Li - School of Molecular Biosciences, Center for Reproductive Biology, Washington State University, Pullman, Washington, USA
Kwan Hee Kim
Publication Details: Journal of andrology, Vol.24(5), pp.653-660
Publisher: United States
Grant note: HD-40456 / NICHD NIH HHS
Identifiers: 99900547977201842
Language: English
Resource Type: Journal article

Effects of ethanol on embryonic and neonatal rat testes in organ cultures

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