Journal article
Effects of exercise and antioxidant supplementation on endothelial gene expression
International journal of cardiology, Vol.158(1), pp.59-65
06/28/2012
Handle:
https://hdl.handle.net/2376/105831
PMID: 21295869
Abstract
The molecular mechanisms of exercise training induced cardiovascular protection are poorly understood. There is growing evidence that reactive oxygen species may be involved in a number of these adaptations and that antioxidants may be used to investigate this effect.
To determine the effects of exercise training and/or antioxidant supplementation on myocardial endothelium and vascular endothelium gene expression.
Male Wistar rats were divided into four groups: i) control; ii) exercise trained (90min of treadmill running 4d per week, 14weeks); iii) antioxidant-supplemented (α-tocopherol 1000IUkg−1 diet and α-lipoic acid 1.6gkg−1 diet, mixed with rat chow) and iv) exercise trained and antioxidant-supplemented.
cDNA microarray analysis showed diverse expression changes in both left ventricular and coronary artery endothelial cells. In particular, RT-PCR analysis showed that a gene involved in cardiovascular disease progression, Ras homolog gene family member A, was down-regulated by exercise, and up-regulated by antioxidant supplementation in left ventricular endothelial cells. Furthermore, an important gene involved in inflammation, IL-6, was down-regulated by all treatments.
Exercise training and/or antioxidant supplementation affects cardiac endothelial cell gene expression, and their effects on genes such as ras homolog gene family member A and IL-6 provides insight into the molecular mechanisms of their influences on cardiovascular diseases.
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Details
- Title
- Effects of exercise and antioxidant supplementation on endothelial gene expression
- Creators
- Aya Matsumoto - School of Human Movement Studies, The University of Queensland, Brisbane, Queensland, AustraliaSteven R Mason - Department of Biochemistry and Molecular Biology, The University of Queensland, Brisbane, Queensland, AustraliaTraute Flatscher-Bader - Department of Biochemistry and Molecular Biology, The University of Queensland, Brisbane, Queensland, AustraliaLeigh C Ward - Department of Biochemistry and Molecular Biology, The University of Queensland, Brisbane, Queensland, AustraliaSusan A Marsh - Program in Nutrition and Exercise Physiology, Washington State University, Spokane, WA, USAPeter A Wilce - Department of Biochemistry and Molecular Biology, The University of Queensland, Brisbane, Queensland, AustraliaRobert G Fassett - School of Human Movement Studies, The University of Queensland, Brisbane, Queensland, AustraliaJudy B de Haan - Oxidative Stress Laboratory, Diabetic Complications Division, Baker IDI Heart and Diabetes Institutes, Melbourne, AustraliaJeff S Coombes - School of Human Movement Studies, The University of Queensland, Brisbane, Queensland, Australia
- Publication Details
- International journal of cardiology, Vol.158(1), pp.59-65
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Elsevier Ireland Ltd
- Grant note
- The University of Queensland Research Development Grant Renal Research Tasmania
- Identifiers
- 99900546796001842
- Language
- English
- Resource Type
- Journal article