Journal article
Effects of hemicellulose and lignin on enzymatic hydrolysis of cellulose from dairy manure
Applied biochemistry and biotechnology, Vol.121-124(1), pp.1017-1030
2005
Handle:
https://hdl.handle.net/2376/102143
PMID: 15930578
Abstract
This study focused on the effect of hemicellulose and lignin on enzymatic hydrolysis of dairy manure and hydrolysis process optimization to improve sugar yield. It was found that hemicellulose and lignin in dairy manure, similar to their role in other lignocellulosic material, were major resistive factors to enzymatic hydrolysis and that the removal of either of them, or for best performance, both of them, improved the enzymatic hydrolysis of manure cellulose. This result combined with scanning electron microscope (SEM) pictures further proved that the accessibility of cellulose to cellulase was the most important feature to the hydrolysis. Quantitatively, fed-batch enzymatic hydrolysis of fiber without lignin and hemicellulose had a high glucose yield of 52% with respect to the glucose concentration of 17 g/L at a total enzyme loading of 1300 FPU/L and reaction time of 160 h, which was better than corresponding batch enzymatic hydrolysis.
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Details
- Title
- Effects of hemicellulose and lignin on enzymatic hydrolysis of cellulose from dairy manure
- Creators
- Wei Liao - Department of Biological Systems Engineering and Center for Multiphase Environmental Research, and, Washington State University, Pullman, WA 99163, USA. wliao@mail.wsu.eduZhiyou WenSharon HurleyYan LiuChuanbin LiuShulin Chen
- Publication Details
- Applied biochemistry and biotechnology, Vol.121-124(1), pp.1017-1030
- Academic Unit
- Biological Systems Engineering, Department of; Plant Pathology, Department of
- Publisher
- United States
- Identifiers
- 99900546796101842
- Language
- English
- Resource Type
- Journal article