Journal article
Effects of minerals on sporulation and heat resistance of Clostridium sporogenes
International journal of food microbiology, Vol.128(2), pp.385-389
2008
Handle:
https://hdl.handle.net/2376/106268
PMID: 18986726
Abstract
In this study, various mineral supplements, such as chloride salts (CaCl
2, MgCl
2, MnCl
2, FeCl
2 and KCl) supplying cations and calcium salts (CaCl
2, CaCO
3, CaSO
4, Ca(OH)
2 and CaHPO
4) supplying anions, were tested if they could stimulate the sporulation of
Clostridium sporogenes, a surrogate microorganism for
C. botulinum. Of the cations tested, the addition of CaCl
2 showed a slightly, but not significantly, greater increase in spore levels within 3 weeks of incubation, compared to that of the other cations. The optimum concentration of CaCl
2 was 0.5%, which yielded nearly 10
4 CFU/ml of spores. Of the anions tested, CaCO
3 promoted sporulation within one week, which was the most effective compound for promoting rapid sporulation among the minerals tested. CaSO
4 produced a pattern of sporulation similar to that of CaCl
2. While CaHPO
4 resulted in the maximum production of spores after 4 weeks, Ca(OH)
2 failed to induce sporulation. With an optimized concentration of 0.5% CaCO
3, the spore yield was approximately 10
5 CFU/ml. The spores prepared in sporulation medium with CaCO
3 (pH 5.0) had slightly, but not significantly, higher
D values than those produced with CaCl
2 (pH 5.0) at temperatures ranging from 113 to 121 °C. However, no significant differences were observed in
Z values (both 10.76 °C). In a large scale spore production,
D
121 °C values of the spore crops prepared with CaCl
2 and CaCO
3 and resuspended in phosphate buffer (pH 7.0) were found to be both 0.92 min. In conclusion, our data suggest that CaCO
3 is highly effective in reducing sporulation time as well as enhancing heat resistance.
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Details
- Title
- Effects of minerals on sporulation and heat resistance of Clostridium sporogenes
- Creators
- Jae-Hyung Mah - Department of Biological Systems Engineering, Washington State University, Pullman, Washington 99164-6120, USADong-Hyun Kang - Department of Food Science and Human Nutrition, Washington State University, Pullman, Washington 99164-6376, USAJuming Tang - Department of Biological Systems Engineering, Washington State University, Pullman, Washington 99164-6120, USA
- Publication Details
- International journal of food microbiology, Vol.128(2), pp.385-389
- Academic Unit
- Biological Systems Engineering, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900546970701842
- Language
- English
- Resource Type
- Journal article