Journal article
Effects of storage methods on the recovery of Mycoplasma species from milk samples
Veterinary microbiology, Vol.144(1), pp.210-213
2010
Handle:
https://hdl.handle.net/2376/109193
PMID: 20053506
Abstract
Mycoplasma species are fastidious microorganisms causing mastitis in dairy cows. Storage by freezing milk samples affects their viability. The purpose of this study was to compare the effect of alternative storage methods on their recoverability. In Experiment I, mycoplasma counts from fresh milk samples were compared to those same samples stored for 1, 3, and 5 days at refrigerated (5
°C) temperatures. Experiment II was done to compare the mycoplasma counts of fresh milk samples with those stored frozen (−20
°C) with addition of 0%, 10%, 30% and 50% glycerol (v/v). Two strains of each of 5 species:
M. bovis,
M. californicum,
M. bovigenitalium,
M. canadense and
M. alkalescens, were selected and inoculated into bulk tank milk free of this pathogen. Compared to those in fresh milk samples, counts were approximately reduced by: 0.3
log
10
CFU/ml in 5 day refrigerated milk (
P
<
0.05) and by 1.0
log
10
CFU/ml in milk frozen without glycerol (
P
<
0.05). Addition of glycerol (10% and 30%, v/v) to milk samples increased the number of recovered
Mycoplasma by up to 0.4
log
10
CFU/ml in frozen milk samples (
P
<
0.05). No significant interactions were detected between either
Mycoplasma species or starting concentration and survival as effected by storage method. Refrigerating milk samples for 5 days and freezing milk samples lowers the number of recovered
Mycoplasma species
. The addition of glycerol to achieve 10% and 30% v/v solutions improves the recovery of
Mycoplasma species from frozen milk samples. To maximize detection of this pathogen, fresh milk samples should be cultured without storage.
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Details
- Title
- Effects of storage methods on the recovery of Mycoplasma species from milk samples
- Creators
- S Boonyayatra - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman 99164, WA 00164-7060, USAL.K Fox - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman 99164, WA 00164-7060, USAT.E Besser - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99164, WA, USAA Sawant - Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman 99164, WA, USAJ.M Gay - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Washington State University, Pullman 99164, WA 00164-7060, USA
- Publication Details
- Veterinary microbiology, Vol.144(1), pp.210-213
- Academic Unit
- Veterinary Clinical Sciences, Department of; Veterinary Microbiology and Pathology, Department of
- Publisher
- Elsevier B.V
- Identifiers
- 99900547174301842
- Language
- English
- Resource Type
- Journal article