Journal article
Effects of sugar inhibition on cellulases and beta-glucosidase during enzymatic hydrolysis of softwood substrates
Applied biochemistry and biotechnology, Vol.113-116(1), pp.1115-1126
2004
Handle:
https://hdl.handle.net/2376/103931
PMID: 15054257
Abstract
A quantitative approach was taken to determine the inhibition effects of glucose and other sugar monomers during cellulase and beta-Glucosidase hydrolysis of two types of cellulosic material: Avicel and acetic acid-pretreated softwood. The increased glucose content in the hydrolysate resulted in a dramatic increase in the degrees of inhibition on both beta-Glucosidase and cellulase activities. Supplementation of mannose, xylose, and galactose during cellobiose hydrolysis did not show any inhibitory effects on beta-Glucosidase activity. However, these sugars were shown to have significant inhibitory effects on cellulase activity during cellulose hydrolysis. Our study suggests that high-substrate consistency hydrolysis with supplementation of hemicellulose is likely to be a practical solution to minimizing end-product inhibition effects while producing hydrolysate with high glucose concentration.
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Details
- Title
- Effects of sugar inhibition on cellulases and beta-glucosidase during enzymatic hydrolysis of softwood substrates
- Creators
- Zhizhuang Xiao - Forest Products Biotechnology, University of British Columbia, 4033-2424 Main Mall, Vancouver BC, V6T 1Z4 CanadaXiao ZhangDavid J GreggJohn N Saddler
- Publication Details
- Applied biochemistry and biotechnology, Vol.113-116(1), pp.1115-1126
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- United States
- Identifiers
- 99900546706801842
- Language
- English
- Resource Type
- Journal article