Journal article
Efficacy of Amphiphilic Core-Shell Nanostructures Encapsulating Gentamicin in an In Vitro Salmonella and Listeria Intracellular Infection Model
Antimicrobial agents and chemotherapy, Vol.54(8), pp.3524-3526
08/2010
Handle:
https://hdl.handle.net/2376/106433
PMCID: PMC2916305
PMID: 20516284
Abstract
Core-shell nanostructures with nonionic amphiphilic shells and ionic cores encapsulating gentamicin were designed for therapy against intracellular pathogens, including
Salmonella
and
Listeria
. Flow cytometry and confocal microscopy showed that their uptake into J774A.1 macrophages proceeded mainly by fluid-phase endocytosis and clathrin-mediated pathways. The nanostructures were nontoxic
in vitro
at doses of 50 to 250 μg/ml, and they significantly reduced the amounts of intracellular
Salmonella
(0.53 log) and
Listeria
(3.16 log), thereby suggesting effective transport into the cells.
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Details
- Title
- Efficacy of Amphiphilic Core-Shell Nanostructures Encapsulating Gentamicin in an In Vitro Salmonella and Listeria Intracellular Infection Model
- Creators
- A Ranjan - Department of Large Animal Clinical SciencesN Pothayee - Department of Large Animal Clinical SciencesT. P Vadala - Department of Large Animal Clinical SciencesM. N Seleem - Department of Large Animal Clinical SciencesE Restis - Department of Large Animal Clinical SciencesN Sriranganathan - Department of Large Animal Clinical SciencesJ. S Riffle - Department of Large Animal Clinical SciencesR Kasimanickam - Department of Large Animal Clinical Sciences
- Publication Details
- Antimicrobial agents and chemotherapy, Vol.54(8), pp.3524-3526
- Academic Unit
- Veterinary Clinical Sciences, Department of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900546972001842
- Language
- English
- Resource Type
- Journal article