Electrically polarized biphasic calcium phosphates: adsorption and release of bovine serum albumin

Solaiman Tarafder; Shashwat Banerjee; Amit Bandyopadhyay; Susmita Bose

doi:10.1021/la101851f

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Electrically polarized biphasic calcium phosphates: adsorption and release of bovine serum albumin

Journal article

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Electrically polarized biphasic calcium phosphates: adsorption and release of bovine serum albumin

Solaiman Tarafder, Shashwat Banerjee, Amit Bandyopadhyay and Susmita Bose

Langmuir, Vol.26(22), pp.16625-16629

11/16/2010

DOI: https://doi.org/10.1021/la101851f

Handle:

https://hdl.handle.net/2376/110135

PMCID: PMC2979312

PMID: 20939493

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Abstract

Animals

Adsorption

Electricity

Protein Structure, Secondary

Cattle

Surface Properties

Calcium Phosphates - chemistry

Serum Albumin, Bovine - chemistry

Electric Conductivity

Drug Carriers - chemistry

In this study, we applied electrical polarization technique to increase adsorption and control protein release from biphasic calcium phosphate (BCP). Three different biphasic calcium phosphate (BCP) composites, with hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP), were processed and electrically polarized. Our study showed that stored charge was increased in the composites with the increase in HAp percentage. Adsorption of bovine serum albumin (BSA), as a model protein, on the poled as well as unpoled surfaces of the composites was studied. The highest amount of BSA adsorption was obtained on positively poled surfaces of each composite. Adsorption isotherm study suggested a multilayer adsorption of BSA on the BCP composites. The effect of electrical polarization on BSA release kinetics from positively charged BCP surfaces was studied. A gradual increase in percent BSA release from positively charged BCP surfaces with decreasing stored charge was observed. Our study showed that the BCP based composites have the potential to be used as a drug or growth factor delivery vehicle.

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Title: Electrically polarized biphasic calcium phosphates: adsorption and release of bovine serum albumin
Creators: Solaiman Tarafder - W. M. Keck Biomedical Materials Lab, School of Mechanical and Materials Engineering, Washington State University, Pullman, Washington 99164-2920, USA
Shashwat Banerjee
Amit Bandyopadhyay
Susmita Bose
Publication Details: Langmuir, Vol.26(22), pp.16625-16629
Academic Unit: Mechanical and Materials Engineering, School of
Publisher: United States
Grant note: R0-EB-007351 / NIBIB NIH HHS R01 EB007351-01A1 / NIBIB NIH HHS R01 EB007351 / NIBIB NIH HHS
Identifiers: 99900547145501842
Language: English
Resource Type: Journal article