Journal article
Electrophoretic and Western blot analyses of the lipopolysaccharide and glycocalyx of Flavobacterium psychrophilum
Fish & shellfish immunology, Vol.23(4), pp.770-780
2007
Handle:
https://hdl.handle.net/2376/107264
PMID: 17420143
Abstract
Flavobacterium psychrophilum is the aetiological agent of bacterial coldwater disease (CWD) and rainbow trout fry syndrome (RTFS) and it has emerged as one of the most significant bacterial pathogens in salmonid aquaculture worldwide. Previous studies have suggested that the O-polysaccharide (O-PS) component of the lipopolysaccharide (LPS) of
F. psychrophilum is highly immunogenic and may be involved in eliciting a protective immune response in rainbow trout (
Oncorhynchus mykiss Walbaum). In the present study, SDS-PAGE and Western blotting techniques were used to analyse the carbohydrate antigens of
F. psychrophilum. Our analysis identified two distinct carbohydrate-banding patterns. One banding pattern corresponds with LPS, and we hypothesise that the other carbohydrate-banding pattern is that of the loosely associated glycocalyx of
F. psychrophilum. Electron microscopy of
F. psychrophilum cells immunogold labelled with a monoclonal antibody specific for this banding pattern supports this hypothesis as the outermost layer of the bacterium was heavily labelled. This is a significant finding because the immunogenic antigens that have been referred to as the O-PS of LPS, and implicated as potential vaccine candidate antigens, appear to be components of the glycocalyx of
F. psychrophilum. This research suggests that the glycocalyx of
F. psychrophilum may be an important antigen to consider for the development of a vaccine to control CWD and RTFS.
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Details
- Title
- Electrophoretic and Western blot analyses of the lipopolysaccharide and glycocalyx of Flavobacterium psychrophilum
- Creators
- Benjamin R LaFrentz - Department of Fish and Wildlife Resources and the Aquaculture Research Institute, University of Idaho, P.O. Box 441136, Moscow, ID 83844-1136, USANicole M Lindstrom - Department of Fish and Wildlife Resources and the Aquaculture Research Institute, University of Idaho, P.O. Box 441136, Moscow, ID 83844-1136, USAScott E LaPatra - Clear Springs Foods, Inc., Research Division, P.O. Box 712, Buhl, ID 83316, USADouglas R Call - Department of Veterinary Microbiology and Pathology, Washington State University, 402 Bustad Hall, Pullman, WA 99164-7040, USAKenneth D Cain - Department of Fish and Wildlife Resources and the Aquaculture Research Institute, University of Idaho, P.O. Box 441136, Moscow, ID 83844-1136, USA
- Publication Details
- Fish & shellfish immunology, Vol.23(4), pp.770-780
- Academic Unit
- Center for Reproductive Biology; Paul G. Allen School for Global Animal Health
- Publisher
- Elsevier Ltd
- Identifiers
- 99900547152901842
- Language
- English
- Resource Type
- Journal article