Electrophoretic field gradient focusing with on-column detection by fluorescence quenching

Richard J Ansell; Pilar González Tuñón; Yating Wang; Peter Myers; Cornelius F Ivory; Jeffrey N Keen; John B C Findlay

doi:10.1039/b815446e

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Electrophoretic field gradient focusing with on-column detection by fluorescence quenching

Journal article

Open access

Electrophoretic field gradient focusing with on-column detection by fluorescence quenching

Richard J Ansell, Pilar González Tuñón, Yating Wang, Peter Myers, Cornelius F Ivory, Jeffrey N Keen and John B C Findlay

Analyst (London), Vol.134(2), pp.226-229

02/2009

DOI: https://doi.org/10.1039/b815446e

Handle:

https://hdl.handle.net/2376/108111

PMID: 19173041

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Abstract

Animals

Electromagnetic Fields

Isoelectric Focusing - instrumentation

Isoelectric Focusing - methods

Proteins - analysis

Equipment Design

Fluorescent Dyes

Native, uncoloured, proteins can be focused in a column containing a fluorescent packing material, using hydrodynamic flow and a counteracting non-linear electric field, and imaged along the length of the channel by fluorescence quenching.

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Details

Title: Electrophoretic field gradient focusing with on-column detection by fluorescence quenching
Creators: Richard J Ansell - School of Chemistry, University of Leeds, Leeds, UK. chmrja@leeds.ac.uk
Pilar González Tuñón
Yating Wang
Peter Myers
Cornelius F Ivory
Jeffrey N Keen
John B C Findlay
Publication Details: Analyst (London), Vol.134(2), pp.226-229
Academic Unit: Chemical Engineering and Bioengineering, School of
Publisher: England
Grant note: BB/C006771/1 / Biotechnology and Biological Sciences Research Council
Identifiers: 99900547346101842
Language: English
Resource Type: Journal article