Journal article
Endogenous nonneuronal modulators of synaptic transmission control cortical slow oscillations in vivo
Proceedings of the National Academy of Sciences - PNAS, Vol.106(35), pp.15037-15042
09/01/2009
Handle:
https://hdl.handle.net/2376/106064
PMCID: PMC2736412
PMID: 19706442
Abstract
Gliotransmission, the release of molecules from astrocytes, regulates neuronal excitability and synaptic transmission in situ. Whether this process affects neuronal network activity in vivo is not known. Using a combination of astrocyte-specific molecular genetics, with in vivo electrophysiology and pharmacology, we determined that gliotransmission modulates cortical slow oscillations, a rhythm characterizing nonrapid eye movement sleep. Inhibition of gliotransmission by the expression of a dominant negative SNARE domain in astrocytes affected cortical slow oscillations, reducing the duration of neuronal depolarizations and causing prolonged hyperpolarizations. These network effects result from the astrocytic modulation of intracortical synaptic transmission at two sites: a hypofunction of postsynaptic NMDA receptors, and by reducing extracellular adenosine, a loss of tonic A1 receptor-mediated inhibition. These results demonstrate that rhythmic brain activity is generated by the coordinated action of the neuronal and glial networks.
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Details
- Title
- Endogenous nonneuronal modulators of synaptic transmission control cortical slow oscillations in vivo
- Creators
- Tommaso Fellin - Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104Michael M Halassa - Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104Miho Terunuma - Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104Francesca Succol - Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104Hajime Takano - Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104Marcos Frank - Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104Stephen J Moss - Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104Philip G Haydon - Silvio Conte Center for Integration at the Tripartite Synapse, Department of Neuroscience, University of Pennsylvania School of Medicine, Philadelphia, PA 19104
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.106(35), pp.15037-15042
- Academic Unit
- Biomedical Sciences, Department of
- Publisher
- National Academy of Sciences
- Identifiers
- 99900546828901842
- Language
- English
- Resource Type
- Journal article