Engineering of an L-arabinose metabolic pathway in Rhodococcus jostii RHA1 for biofuel production

Xiaochao Xiong; Xi Wang; Shulin Chen

doi:10.1007/s10295-016-1778-y

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Engineering of an L-arabinose metabolic pathway in Rhodococcus jostii RHA1 for biofuel production

Journal article

Peer reviewed

Engineering of an L-arabinose metabolic pathway in Rhodococcus jostii RHA1 for biofuel production

Xiaochao Xiong, Xi Wang and Shulin Chen

Journal of industrial microbiology & biotechnology, Vol.43(7), pp.1017-1025

07/2016

DOI: https://doi.org/10.1007/s10295-016-1778-y

Handle:

https://hdl.handle.net/2376/111260

PMID: 27143134

Abstract

Genes, Bacterial

Industrial Microbiology

Protein Engineering - methods

Bacterial Proteins - genetics

Biomass

Triglycerides - metabolism

Proteins - genetics

Metabolic Networks and Pathways - genetics

Proteins - metabolism

Rhodococcus - metabolism

Escherichia coli - genetics

Rhodococcus - genetics

Arabinose - metabolism

Escherichia coli - metabolism

Bacterial Proteins - metabolism

Culture Media - chemistry

Diacylglycerol O-Acyltransferase - metabolism

Gene Expression Regulation, Bacterial

Biofuels - microbiology

The oleaginous bacterium, Rhodococcus jostii RHA1 has attracted considerable attention due to its capability to accumulate significant levels of triacylglycerol as renewable hydrocarbon. To enable the strain to utilize arabinose derived from lignocellulosic biomass, the metabolic pathway of L-arabinose utilization was introduced into R. jostii RHA1 by heterogenous expression of the operon, araBAD from Escherichia coli. The results showed that recombinant bearing araBAD could grow on L-arabinose as the sole carbon source, and additional expression of araFGH encoding the arabinose transporter from E. coli could improve the cell biomass yield from high contents of arabinose. We further increased the content of lipid produced from arabinose in the recombinants from 47.9 to 56.8 % of the cell dry weight (CDW) by overexpression of a gene, atf1 encoding a diglyceride acyltransferase from R. opacus PD630. This work demonstrated the feasibility of producing lipid from arabinose by genetic modification of the rhodococci strain.

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Title: Engineering of an L-arabinose metabolic pathway in Rhodococcus jostii RHA1 for biofuel production
Creators: Xiaochao Xiong - Department of Biological Systems Engineering, Washington State University, L.J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA
Xi Wang - Department of Biological Systems Engineering, Washington State University, L.J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA
Shulin Chen - Department of Biological Systems Engineering, Washington State University, L.J. Smith Hall, P.O. Box 646120, Pullman, WA, 99164-6120, USA. chens@wsu.edu
Publication Details: Journal of industrial microbiology & biotechnology, Vol.43(7), pp.1017-1025
Identifiers: 99900583063101842
Language: English
Resource Type: Journal article

Engineering of an L-arabinose metabolic pathway in Rhodococcus jostii RHA1 for biofuel production

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