Journal article
Enhanced protein glutathiolation and oxidative stress in cigarette smokers
Free radical biology & medicine, Vol.36(4), pp.464-470
02/15/2004
Handle:
https://hdl.handle.net/2376/115284
PMID: 14975449
Abstract
There are many functional assays of oxidative damage to DNA, protein, and lipids but few reliable markers of chronic oxidative stress. The glutathiolation of proteins at key Cys residues is considered an important redox-sensitive, posttranslational signaling mechanism in the regulation of critical cellular functions. To determine whether protein bound glutathione (GSSP) is a sensitive indicator of oxidative stress, red blood cell and plasma concentrations were measured and compared between smokers and nonsmokers. In a community-based study conducted in Westchester County, New York, USA, blood samples were obtained from 354 cigarette smokers and 97 never smokers. The mean concentration of blood GSSP (micromol/L) was 32% higher in cigarette smokers and 43% higher when standardized by hemoglobin concentrations (p <.01). Plasma GSSP levels were also 20% higher in smokers than in nonsmokers (p <.001). The relationship was dose-dependent, with blood GSSP levels significantly correlated with cigarettes smoked per day, plasma cotinine, and plasma thiocyanate (r values ranged from .25 to .40). In smokers, there were no significant differences in GSSP and GSH levels by GSTM1 or GSTM3 genotype. Intraindividual variation in blood samples, as determined by taking serial samples over a 2-week period, was low (CV = 12.1%, n = 8). GSSP levels are stable over time but increase in response to the abundant free radicals in cigarette smoke. These findings support the use of GSSP as a sensitive biomarker of oxidative stress.
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Details
- Title
- Enhanced protein glutathiolation and oxidative stress in cigarette smokers
- Creators
- Joshua E Muscat - Institute for Cancer Prevention, American Health Foundation Cancer Center, Valhalla, NY 10595, USAWayne KleinmanStephen ColosimoAllison MuirPhilip LazarusJong ParkJohn P Richie, Jr
- Publication Details
- Free radical biology & medicine, Vol.36(4), pp.464-470
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- United States
- Grant note
- F32 CA073173 / NCI NIH HHS CA17613 / NCI NIH HHS R01 DE013158 / NIDCR NIH HHS CA68384 / NCI NIH HHS P01 CA068384 / NCI NIH HHS
- Identifiers
- 99900547694101842
- Language
- English
- Resource Type
- Journal article