Journal article
Enhancement of linoleic acid content stimulates astaxanthin esterification in Coelastrum sp
Bioresource technology, Vol.300, pp.122649-122649
03/2020
Handle:
https://hdl.handle.net/2376/118018
PMID: 31896045
Abstract
[Display omitted]
•With half of astaxanthin was free-type, a special alga Coelastrum sp. was studied.•Astaxanthin esterification was stimulated with linoleic acid and ethanol treatment.•Ethanol enhanced transcription of fad2, which promoted oleic acid to linoleic acid.•Linoleic acid and ethanol increased the astaxanthin ester contents by 282% and 142%.
Most natural astaxanthin is fatty acid-esterified in microalgae to prevent oxidation. However, the factors influencing astaxanthin esterification (AE) are poorly understood. In this study, obstacles to AE in Coelastrum sp. HA-1 were investigated. Only half of the astaxanthin molecules in HA-1 were esterified, but AE was stimulated with exogenous linoleic acid (LA) and ethanol treatment. Astaxanthin esters and total astaxanthin (TA) with exogenous LA were elevated to 3.82-fold and 2.18-fold of control levels, respectively. Treatment with 3% (v/v) ethanol enhanced transcription of the Δ12 fatty acid desaturase gene, which caused more oleic acid (OA) to be converted to LA. Furthermore, the contents of astaxanthin esters and TA were 2.42-fold and 1.61-fold control levels, respectively. These findings confirmed that AE was upregulated by increasing LA content. Thus, a large concentration of OA alone does not increase astaxanthin accumulation in HA-1, and a certain amount of LA was necessary for AE.
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Details
- Title
- Enhancement of linoleic acid content stimulates astaxanthin esterification in Coelastrum sp
- Creators
- Zhiyong Liu - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaYuyong Hou - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaChunqing He - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaXuan Wang - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaShulin Chen - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaZhiyong Huang - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, ChinaFangjian Chen - Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
- Publication Details
- Bioresource technology, Vol.300, pp.122649-122649
- Publisher
- Elsevier Ltd
- Identifiers
- 99900582334601842
- Language
- English
- Resource Type
- Journal article