Journal article
Enhancing Isoprene Production by Genetic Modification of the 1-Deoxy-d-Xylulose-5-Phosphate Pathway in Bacillus subtilis
Applied and environmental microbiology, Vol.77(7), pp.2399-2405
04/2011
Handle:
https://hdl.handle.net/2376/112793
PMCID: PMC3067423
PMID: 21296950
Abstract
To enhance the production of isoprene, a volatile 5-carbon hydrocarbon, in the Gram-positive spore-forming rod-shaped bacterium
Bacillus subtilis
, 1-deoxy-
d
-xylulose-5-phosphate synthase (Dxs) and 1-deoxy-
d
-xylulose-5-phosphate reductoisomerase (Dxr) were overexpressed in
B. subtilis
DSM 10. For the strain that overexpresses Dxs, the yield of isoprene was increased 40% over that by the wild-type strain. In the Dxr overexpression strain, the level of isoprene production was unchanged. Overexpression of Dxr together with Dxs showed an isoprene production level similar to that of the Dxs overproduction strain. The effects of external factors, such as stress factors including heat (48°C), salt (0.3 M NaCl), ethanol (1%), and oxidative (0.005% H
2
O
2
) stress, on isoprene production were further examined. Heat, salt, and H
2
O
2
induced isoprene production; ethanol inhibited isoprene production. In addition, induction and repression effects are independent of SigB, which is the general stress-responsive alternative sigma factor of Gram-positive bacteria.
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Details
- Title
- Enhancing Isoprene Production by Genetic Modification of the 1-Deoxy-d-Xylulose-5-Phosphate Pathway in Bacillus subtilis
- Creators
- Junfeng Xue - Bioproducts, Sciences and Engineering Laboratory, Washington State University Tri-Cities, Richland, Washington 99354Birgitte K Ahring - Bioproducts, Sciences and Engineering Laboratory, Washington State University Tri-Cities, Richland, Washington 99354
- Publication Details
- Applied and environmental microbiology, Vol.77(7), pp.2399-2405
- Academic Unit
- Chemical Engineering and Bioengineering, School of
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900547548401842
- Language
- English
- Resource Type
- Journal article