Journal article
Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum
British journal of venereal diseases, Vol.59(2), pp.75-79
04/1983
Handle:
https://hdl.handle.net/2376/116102
PMID: 6339000
Abstract
A highly sensitive enzyme-linked immunosorbent assay was used for the analysis of serum IgG reactivity against specific immunogenic Treponema pallidum proteins. Outer membrane treponemal proteins purified by preparative SDS-polyacrylamide gel electrophoresis were used as antigenic probes at concentrations as low as 100 ng per ml (5 ng per well in microtitre plates). Detection of anti-treponemal antibody was possible using rabbit syphilitic sera diluted to 1/10 000. The sensitivity of the assay was equal to or greater than that detected by radioimmuno-precipitation combined with gel electrophoresis and fluorography techniques and was capable of monitoring host IgG responses throughout the progress of the disease.
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Details
- Title
- Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum
- Creators
- J Morrison-PlummerJ F AldereteJ B Baseman
- Publication Details
- British journal of venereal diseases, Vol.59(2), pp.75-79
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- England
- Identifiers
- 99900547592001842
- Language
- English
- Resource Type
- Journal article