Epidermal jasmonate perception is sufficient for all aspects of jasmonate-mediated male fertility in Arabidopsis

Jeremy B Jewell; John Browse

doi:10.1111/tpj.13131

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Epidermal jasmonate perception is sufficient for all aspects of jasmonate-mediated male fertility in Arabidopsis

Journal article

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Epidermal jasmonate perception is sufficient for all aspects of jasmonate-mediated male fertility in Arabidopsis

Jeremy B Jewell and John Browse

The Plant journal : for cell and molecular biology, Vol.85(5), pp.634-647

03/2016

DOI: https://doi.org/10.1111/tpj.13131

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https://hdl.handle.net/2376/102934

PMID: 26833563

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Abstract

Pollen - metabolism

Flowers - metabolism

Homeodomain Proteins - metabolism

Cell Survival - genetics

Plant Epidermis - genetics

Plant Epidermis - metabolism

Promoter Regions, Genetic - genetics

Arabidopsis Proteins - metabolism

Plants, Genetically Modified

Pollen - genetics

Gene Expression Regulation, Plant

Pollen - drug effects

Cell Survival - drug effects

Arabidopsis Proteins - genetics

Arabidopsis - drug effects

Signal Transduction - genetics

Cyclopentanes - pharmacology

Homeodomain Proteins - genetics

Arabidopsis - metabolism

Arabidopsis - genetics

Microscopy, Confocal

Oxylipins - metabolism

Plant Infertility - genetics

Signal Transduction - drug effects

Oxylipins - pharmacology

Cyclopentanes - metabolism

Luminescent Proteins - genetics

Flowers - drug effects

Mutation

Plant Growth Regulators - metabolism

Flowers - genetics

Plant Growth Regulators - pharmacology

Luminescent Proteins - metabolism

Jasmonate (JA) signaling is essential for several environmental responses and reproductive development in many plant species. In Arabidopsis thaliana, the most obvious phenotype of JA biosynthetic and perception mutants is profound sporophytic male sterility characterized by failure of stamen filament elongation, severe delay of anther dehiscence and pollen inviability. The site of action of JA in the context of reproductive development has been discussed, but the ideas have not been tested experimentally. To this end we used targeted expression of a COI1-YFP transgene in the coi1-1 mutant background. As COI1 is an essential component of the JA co-receptor complex, the null coi1-1 mutant is male sterile due to lack of JA perception. We show that expression of COI1-YFP in the epidermis of the stamen filament and anther in coi1 mutant plants is sufficient to rescue filament elongation, anther dehiscence and pollen viability. In contrast, filament expression alone or expression in the tapetum do not restore dehiscence and pollen viability. These results demonstrate that epidermal JA perception is sufficient for anther function and pollen viability, and suggest the presence of a JA-dependent non-autonomous signal produced in the anther epidermis to synchronize both anther dehiscence and pollen maturation.

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Title: Epidermal jasmonate perception is sufficient for all aspects of jasmonate-mediated male fertility in Arabidopsis
Creators: Jeremy B Jewell - Institute of Biological Chemistry, Washington State University, Pullman, WA, 99164-6340, USA
John Browse - Institute of Biological Chemistry, Washington State University, Pullman, WA, 99164-6340, USA
Publication Details: The Plant journal : for cell and molecular biology, Vol.85(5), pp.634-647
Academic Unit: Biological Chemistry, Institute of
Publisher: England
Identifiers: 99900546680001842
Language: English
Resource Type: Journal article