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Journal article
Epigenetic Transgenerational Inheritance of Altered Sperm Histone Retention Sites
Scientific reports, Vol.8(Mar (E-published)), pp.1-10
03/28/2018
PMCID: PMC5871750
PMID: 29593303
Abstract
A variety of environmental toxicants and factors have been shown to induce the epigenetic transgenerational inheritance of disease and phenotypic variation. Epigenetic alterations in the germline (sperm or egg) are required to transmit transgenerational phenotypes. The current study was designed to investigate the potential role of histones in sperm to help mediate the epigenetic transgenerational inheritance. The agricultural fungicide vinclozolin and the pesticide DDT (dichlorodiphenyltrichloroethane) were independently used to promote the epigenetic transgenerational inheritance of disease. Purified cauda epididymal sperm were collected from the transgenerational F3 generation control and exposure lineage male rats for histone analysis. A reproducible core of histone H3 retention sites was observed using an H3 chromatin immunoprecipitation (ChIP-Seq) analysis in control lineage sperm. Interestingly, the same core group of H3 retention sites plus additional differential histone retention sites (DHRs) were observed in the F3 generation exposure lineage sperm. Although new histone H3 retention sites were observed, negligible change in histone modification (methylation of H3K27me3) was observed between the control and exposure lineages. Observations demonstrate that in addition to alterations in sperm DNA methylation and ncRNA previously identified, the induction of differential histone retention sites (DHRs) also appear to be involved in environmentally induced epigenetic transgenerational inheritance.
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Details
- Title
- Epigenetic Transgenerational Inheritance of Altered Sperm Histone Retention Sites
- Creators
- Millissia Ben Maamar - Washington State UniversityIngrid Sadler-Riggleman - Washington State UniversityDaniel Beck - Washington State UniversityMichael K. Skinner - Washington State University
- Publication Details
- Scientific reports, Vol.8(Mar (E-published)), pp.1-10
- Academic Unit
- Biological Sciences, School of
- Publisher
- Springer Nature
- Number of pages
- 10
- Grant note
- 50183 / John Templeton Foundation ES012974-10 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA R01ES012974 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Identifiers
- 99901080304501842
- Language
- English
- Resource Type
- Journal article