Epitope specificity is critical for high and moderate avidity cytotoxic T lymphocytes associated with control of viral load and clinical disease in horses with equine infectious anemia virus

Robert H Mealey; Baoshan Zhang; Steven R Leib; Matt H Littke; Travis C McGuire

doi:10.1016/S0042-6822(03)00344-1

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Epitope specificity is critical for high and moderate avidity cytotoxic T lymphocytes associated with control of viral load and clinical disease in horses with equine infectious anemia virus

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Epitope specificity is critical for high and moderate avidity cytotoxic T lymphocytes associated with control of viral load and clinical disease in horses with equine infectious anemia virus

Robert H Mealey, Baoshan Zhang, Steven R Leib, Matt H Littke and Travis C McGuire

Virology (New York, N.Y.), Vol.313(2), pp.537-552

09/01/2003

DOI: https://doi.org/10.1016/S0042-6822(03)00344-1

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https://hdl.handle.net/2376/112840

PMCID: PMC3342690

PMID: 12954220

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https://doi.org/10.1016/S0042-6822(03)00344-1View

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Abstract

Glycoproteins - genetics

T-Lymphocytes, Cytotoxic - immunology

Amino Acid Sequence

Epitope Mapping

Viral Envelope Proteins - genetics

Equine Infectious Anemia - diagnosis

Fever - veterinary

Viral Load - veterinary

Molecular Sequence Data

RNA, Viral - analysis

Disease Progression

Equine Infectious Anemia - virology

Infectious Anemia Virus, Equine - chemistry

Glycoproteins - immunology

Sequence Alignment

Animals

Horses

Equine Infectious Anemia - immunology

Immunologic Memory

Viral Envelope Proteins - immunology

Epitopes, T-Lymphocyte - immunology

Thrombocytopenia - veterinary

Equine infectious anemia virus (EIAV) is a lentivirus that causes persistent infections in horses. We hypothesized that high-avidity CTL specific for nonvariable epitopes might be associated with low viral load and minimal disease in EIAV-infected horses. To test this hypothesis, memory CTL (CTLm) responses were analyzed in two infected horses with high plasma viral loads and recurrent disease (progressors), and in two infected horses with low-to-undetectable viral loads and mild disease (nonprogressors). High-avidity CTLm in one progressor recognized an envelope gp90 epitope, and the data documented for the first time in EIAV that viral variation led to CTL escape. Each of the nonprogressors had high-to-moderate avidity CTLm directed against epitopes within Rev, including the nuclear export and nuclear localization domains. These results suggested that the epitope specificity of high- and moderate-avidity CTLm was an important determinant for disease outcome in the EIAV-infected horses examined.

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Title: Epitope specificity is critical for high and moderate avidity cytotoxic T lymphocytes associated with control of viral load and clinical disease in horses with equine infectious anemia virus
Creators: Robert H Mealey - Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164-7040, USA. rhm@vetmed.wsu.edu
Baoshan Zhang
Steven R Leib
Matt H Littke
Travis C McGuire
Publication Details: Virology (New York, N.Y.), Vol.313(2), pp.537-552
Academic Unit: Veterinary Clinical Sciences, Department of
Publisher: United States
Grant note: AI24291 / NIAID NIH HHS AI01575 / NIAID NIH HHS K08 AI001575 / NIAID NIH HHS
Identifiers: 99900548113401842
Language: English
Resource Type: Journal article