Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen germination and vegetative growth in Arabidopsis

Carsten Bernard; Michaela Traub; H-Henning Kunz; Stefanie Hach; Oliver Trentmann; Torsten Möhlmann

doi:10.1093/jxb/err183

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Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen germination and vegetative growth in Arabidopsis

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Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen germination and vegetative growth in Arabidopsis

Carsten Bernard, Michaela Traub, H-Henning Kunz, Stefanie Hach, Oliver Trentmann and Torsten Möhlmann

Journal of experimental botany, Vol.62(13), pp.4627-4637

08/2011

DOI: https://doi.org/10.1093/jxb/err183

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https://hdl.handle.net/2376/115244

PMCID: PMC3170557

PMID: 21642237

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Abstract

Equilibrative Nucleoside Transport Proteins - metabolism

Arabidopsis - growth & development

RNA, Messenger - metabolism

Arabidopsis Proteins - metabolism

Organ Specificity - genetics

Extracellular Space - metabolism

RNA Interference

Adenosine Triphosphate - metabolism

Pollen - genetics

Gene Expression Regulation, Plant

Germination - physiology

Cyclic AMP - metabolism

Pollen - physiology

Pollen - growth & development

Arabidopsis Proteins - genetics

RNA, Messenger - genetics

Equilibrative Nucleoside Transporter 1 - genetics

Equilibrative Nucleoside Transporter 1 - metabolism

Mutation - genetics

Subcellular Fractions - metabolism

Protein Transport

Arabidopsis - genetics

Pollen - anatomy & histology

Models, Biological

Intracellular Space - metabolism

Adenosine - metabolism

Vacuoles - metabolism

Equilibrative Nucleoside Transport Proteins - genetics

ENT1 of Arabidopsis thaliana was the first member of the equilibrative nucleoside transporter (ENT) family to be identified in plants and characterized as a cellular, high-affinity nucleoside importer. Evidence is presented here for a tonoplast localization of ENT1 based on proteome data and Western blot analyses. Increased export of adenosine from reconstituted tonoplast preparations from 35S:ENT1 mutants compared with those from the wild type and ENT1-RNAi mutants support this view. Furthermore, increased vacuolar adenosine and vacuolar 2'3'-cAMP (an intermediate of RNA catabolism) contents in ENT1-RNAi mutants, but decreased contents of these metabolites in 35S:ENT1 over-expresser mutants, were observed. An up-regulation of the salvage pathway was detected in the latter mutants, leading to the conclusion that draining the vacuolar adenosine storage by ENT1 over-expression interferes with cellular nucleotide metabolism. As a consequence of the observed metabolic alterations 35S:ENT1 over-expresser mutants exhibited a smaller phenotypic appearance compared with wild-type plants. In addition, ENT1:RNAi mutants exhibited significantly lower in vitro germination of pollen and contained reduced internal and external ATP levels. This indicates that ENT1-mediated nucleosides, especially adenosine transport, is important for nucleotide metabolism, thus influencing growth and pollen germination.

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Title: Equilibrative nucleoside transporter 1 (ENT1) is critical for pollen germination and vegetative growth in Arabidopsis
Creators: Carsten Bernard - Pflanzenphysiologie, Fachbereich Biologie, Universität Kaiserslautern, Erwin-Schrödinger-Strasse, Postfach 3049, D-67663 Kaiserslautern, Germany
Michaela Traub
H-Henning Kunz
Stefanie Hach
Oliver Trentmann
Torsten Möhlmann
Publication Details: Journal of experimental botany, Vol.62(13), pp.4627-4637
Academic Unit: Biological Sciences, School of
Publisher: England
Identifiers: 99900547856001842
Language: English
Resource Type: Journal article