Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses

Travis C McGuire; Steven R Leib; Scott M Lonning; Wei Zhang; Katherine M Byrne; Robert H Mealey

doi:10.1099/0022-1317-81-11-2735

Back

Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses

Journal article

Open access

Peer reviewed

Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses

Travis C McGuire, Steven R Leib, Scott M Lonning, Wei Zhang, Katherine M Byrne and Robert H Mealey

Journal of general virology, Vol.81(Pt 11), pp.2735-2739

11/2000

DOI: https://doi.org/10.1099/0022-1317-81-11-2735

Handle:

https://hdl.handle.net/2376/110188

PMID: 11038386

Files and links (2)

pdf

0812735a391.80 kBDownload View

Open Access

url

https://doi.org/10.1099/0022-1317-81-11-2735View

Published (Version of record) Open

Abstract

Epitopes - immunology

T-Lymphocytes, Cytotoxic - immunology

Antigens, Viral - immunology

Animals

Horses - immunology

Equine Infectious Anemia - immunology

Horses - virology

Infectious Anemia Virus, Equine - immunology

Cytotoxicity, Immunologic

Efficacious lentiviral vaccines designed to induce cytotoxic T lymphocytes (CTL) in outbred populations with a diverse repertoire of MHC class I molecules should contain or express multiple viral proteins. To determine the equine infectious anaemia virus (EIAV) proteins with epitopes most frequently recognized by CTL from seven horses infected for 0.5 to 7 years, retroviral vector-transduced target cells expressing viral proteins were used in CTL assays. Gag p15 was recognized by CTL from 100% of these infected horses. p26 was recognized by CTL from 86%, SU and the middle third of Pol protein were each recognized by 43%, TM by 29%, and S2 by 14%. Based on these results, it is likely that a construct expressing the 359 amino acids constituting p15 and p26 would contain epitopes capable of stimulating CTL in most horses.

Metrics

8 Record Views

Details

Title: Equine infectious anaemia virus proteins with epitopes most frequently recognized by cytotoxic T lymphocytes from infected horses
Creators: Travis C McGuire - Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA
Steven R Leib - Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA
Scott M Lonning - Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA
Wei Zhang - Washington State University
Katherine M Byrne - Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA
Robert H Mealey - Department of Veterinary Microbiology and Pathology1 and Department of Animal Sciences2, Washington State University, Pullman, WA 99164-7040, USA
Publication Details: Journal of general virology, Vol.81(Pt 11), pp.2735-2739
Academic Unit: Department of Veterinary Clinical Sciences
Publisher: England
Grant note: AI24291 / NIAID NIH HHS
Identifiers: 99900546908901842
Language: English
Resource Type: Journal article