Journal article
Establishing a Corky Ringspot Disease Plot for Research Purposes
Journal of nematology, Vol.39(4), pp.313-316
12/2007
Handle:
https://hdl.handle.net/2376/114845
PMCID: PMC2586511
PMID: 19259504
Abstract
A method to establish two experimental corky ringspot disease (CRS) plots that had no prior CRS history is described. CRS is a serious disease of potato in the Pacific Northwest caused by tobacco rattle virus (TRV) and transmitted primarily by
Paratrichodorus allius
. ‘Samsun NN’ tobacco seedlings were inoculated with viruliferous
P. allius
in the greenhouse before they were transplanted into the field soil at the rate of 3,000 plus seedlings/ha. Care was taken to keep soil around plants in the greenhouse and transplants in the field moist to avoid vector mortality. The vector population in the soil of one of the fields was monitored by extraction, examination under microscope and bioassay on tobacco seedlings to ascertain that they were virus carriers. Presence of virus in tobacco bioassay plants was determined by visual symptoms on tobacco leaves and by testing leaves and roots using ELISA. Although TRV transmission was rapid, there was loss of infectivity in the first winter which necessitated a re-inoculation. After two years of planting infected tobacco seedlings, 100% of soil samples collected from this field contained viruliferous
P. allius
. In the second field, all five commercial potato cultivars, known to be susceptible, expressed symptoms of CRS disease indicating that the procedure was successful.
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Details
- Title
- Establishing a Corky Ringspot Disease Plot for Research Purposes
- Creators
- H Mojtahedi - USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350R. A Boydston - USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350J. M Crosslin - USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350C. R Brown - USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350E Riga - USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350T. L Anderson - USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350D Spellman - USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350R. A Quick - USDA-ARS, 24106 N. Bunn Rd., Prosser, WA 99350
- Publication Details
- Journal of nematology, Vol.39(4), pp.313-316
- Academic Unit
- Agricultural, Human, and Natural Resource Sciences, College of
- Publisher
- The Society of Nematologists
- Identifiers
- 99900547611001842
- Language
- English
- Resource Type
- Journal article