Establishment and characterization of a prostate cancer cell line from a prostatectomy specimen for the study of cellular interaction

Ruoxiang Wang; Gina C-Y Chu; Xudong Wang; Jason B Wu; Peizhen Hu; Asha S Multani; Sen Pathak; Haiyen E Zhau; Leland W K Chung

doi:10.1002/ijc.32370

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Establishment and characterization of a prostate cancer cell line from a prostatectomy specimen for the study of cellular interaction

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Establishment and characterization of a prostate cancer cell line from a prostatectomy specimen for the study of cellular interaction

Ruoxiang Wang, Gina C-Y Chu, Xudong Wang, Jason B Wu, Peizhen Hu, Asha S Multani, Sen Pathak, Haiyen E Zhau and Leland W K Chung

International journal of cancer, Vol.145(8), pp.2249-2259

10/15/2019

DOI: https://doi.org/10.1002/ijc.32370

PMID: 31020641

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Abstract

Carcinogenesis

Cell Communication

Cell Line

Cell Line, Transformed

Cell Line, Tumor

Epithelial Cells - cytology

Epithelial Cells - metabolism

Humans

Kallikreins - metabolism

Mesoderm - cytology

Mesoderm - metabolism

Mice

Prostate - cytology

Prostate - metabolism

Prostate-Specific Antigen - metabolism

Prostatectomy

Prostatic Neoplasms - metabolism

Prostatic Neoplasms - pathology

Stromal Cells - cytology

Stromal Cells - metabolism

Transplantation, Heterologous

Tumor Cells, Cultured

Though human prostate cancer (PCa) heterogeneity can best be studied using multiple cell types isolated from clinical specimens, the difficulty of establishing cell lines from clinical tumors has hampered this approach. In this proof-of-concept study, we established a human PCa cell line from a prostatectomy surgical specimen without the need for retroviral transduction. In a previous report, we characterized the stromal cells derived from PCa specimens. Here, we characterized the epithelial cells isolated from the same tumors. Compared to the ease of establishing prostate stromal cell lines, prostatic epithelial cell lines are challenging. From three matched pairs of normal and tumor tissues, we established one new PCa cell line, HPE-15. We confirmed the origin of HPE-15 cells by short tandem repeat microsatellite polymorphism analysis. HPE-15 cells are androgen-insensitive and express marginal androgen receptor, prostate-specific antigen and prostate-specific membrane antigen proteins. HPE-15 expresses luminal epithelial markers of E-cadherin and cytokeratin 18, basal cell markers of cytokeratin 5 and p63 and neuroendocrine marker of chromogranin A. Interestingly, HPE-15 Cells exhibited no tumorigenicity in different strains of immune-deficient mice but can become tumorigenic through interaction with aggressive cancer cell types. HPE-15 cells can thus serve as an experimental model for the study of PCa progression, metastasis and tumor cell dormancy.

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Title: Establishment and characterization of a prostate cancer cell line from a prostatectomy specimen for the study of cellular interaction
Creators: Ruoxiang Wang - Cedars-Sinai Medical Center
Gina C-Y Chu - Cedars-Sinai Medical Center
Xudong Wang - Uro-Oncology Research, Department of Medicine, Cedars-Sinai Medical Center, Los Angeles, CA
Jason B Wu - Washington State University, Pharmaceutical Sciences, Department of
Peizhen Hu - Cedars-Sinai Medical Center
Asha S Multani - The University of Texas MD Anderson Cancer Center
Sen Pathak - The University of Texas MD Anderson Cancer Center
Haiyen E Zhau - Cedars-Sinai Medical Center
Leland W K Chung - Cedars-Sinai Medical Center
Publication Details: International journal of cancer, Vol.145(8), pp.2249-2259
Academic Unit: Pharmacy and Pharmaceutical Sciences, College of
Grant note: R21 CA112330 / NCI NIH HHS P01 CA098912 / NCI NIH HHS
Identifiers: 99900620490001842
Language: English
Resource Type: Journal article