Journal article
Establishment of cell lines from B-cell precursor acute lymphoblastic leukemia
Leukemia, Vol.7(11), pp.1865-1874
11/1993
Handle:
https://hdl.handle.net/2376/106560
PMID: 8231254
Abstract
In an attempt to establish permanent cell lines from children with B-cell precursor acute lymphoblastic leukemia (BCP-ALL), 123 clinical samples from 117 patients were cultured in vitro. Using a method which was successful for the growth of ALL with T-cell phenotype, 3% (2/74) of BCP-ALL samples from patients at diagnosis and 31% (9/29) of BCP-ALL samples from patients at relapse were established as cell lines. However, in most cultures, leukemic cells survived for only a few weeks and the majority of viable cells present after 28 days of culture were esterase-positive mononuclear cells. Based on the hypothesis that mononuclear cells inhibited leukemic cell growth, we evaluated the effect of a monocyte toxin, L-leucine methyl ester (Leu-OMe), on the growth of four frozen BCP-ALL samples. Thawed leukemic cells treated with Leu-OMe, but not untreated control cells, proliferated in three samples and one new cell line was established. Subsequently, when Leu-OMe was added to fresh leukemia cells in culture, leukemic cell lines were grown from 29% (4/14) of samples at diagnosis and 66% (4/6) of relapse samples. Overall, 20 BCP-ALL cell lines were established, all were Epstein-Barr virus (EBV)-negative, and authenticity of each cell line was verified by a direct comparison of the immunophenotype, karyotype, and genotype with the patient's tumor cells. This improved method of cell culture permits a higher success rate of cell line establishment from patients with BCP-ALL thereby aiding in analysis of B-lymphocyte transformation and neoplasia.
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Details
- Title
- Establishment of cell lines from B-cell precursor acute lymphoblastic leukemia
- Creators
- L Q Zhang - University of Chicago Department of Pediatrics, ILP A DownieW R GoodellN R McCabeM M LeBeauR MorganJ SklarS C RaimondiD MileyA Goldberg
- Publication Details
- Leukemia, Vol.7(11), pp.1865-1874
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- England
- Identifiers
- 99900546706401842
- Language
- English
- Resource Type
- Journal article