Journal article
Estrogen receptor α is required for oviductal transport of embryos
The FASEB journal, Vol.31(4), pp.1595-1607
04/2017
Handle:
https://hdl.handle.net/2376/109345
PMCID: PMC5349796
PMID: 28082352
Abstract
Newly fertilized embryos spend the first few days within the oviduct and are transported to the uterus, where they implant onto the uterine wall. An implantation of the embryo before reaching the uterus could result in ectopic pregnancy and lead to maternal death. Estrogen is necessary for embryo transport in mammals; however, the mechanism involved in estrogen-mediated cellular function within the oviduct remains unclear. In this study, we show in mouse models that ciliary length and beat frequency of the oviductal epithelial cells are regulated through estrogen receptor α (ESR1) but not estrogen receptor β (ESR2). Gene profiling indicated that transcripts in the WNT/β-catenin (WNT/CTNNB1) signaling pathway were regulated by estrogen in mouse oviduct, and inhibition of this pathway in a whole oviduct culture system resulted in a decreased embryo transport distance. However, selective ablation of CTNNB1 from the oviductal ciliated cells did not affect embryo transport, possibly because of a compensatory mechanism
intact CTNNB1 in the adjacent secretory cells. In summary, we demonstrated that disruption of estrogen signaling in oviductal epithelial cells alters ciliary function and impairs embryo transport. Therefore, our findings may provide a better understanding of etiology of the ectopic pregnancy that is associated with alteration of estrogen signals.-Li, S., O'Neill, S. R. S., Zhang, Y., Holtzman, M. J., Takemaru, K.-I., Korach, K. S., Winuthayanon, W. Estrogen receptor α is required for oviductal transport of embryos.
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Details
- Title
- Estrogen receptor α is required for oviductal transport of embryos
- Creators
- Shuai Li - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USASofia R S O'Neill - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USAYong Zhang - Pulmonary and Critical Care Medicine, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USAMichael J Holtzman - Pulmonary and Critical Care Medicine, Department of Medicine, Washington University School of Medicine, St. Louis, Missouri, USAKen-Ichi Takemaru - Department of Pharmacological Sciences, Stony Brook University School of Medicine, Stony Brook, New York, USA; andKenneth S Korach - Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, North Carolina, USAWipawee Winuthayanon - School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, Washington, USA; winuthayanonw@vetmed.wsu.edu
- Publication Details
- The FASEB journal, Vol.31(4), pp.1595-1607
- Academic Unit
- Molecular Biosciences, School of
- Publisher
- United States
- Grant note
- R01 HL107493 / NHLBI NIH HHS R01 AI130591 / NIAID NIH HHS ZIA ES070065 / Intramural NIH HHS U19 AI070489 / NIAID NIH HHS
- Identifiers
- 99900547165601842
- Language
- English
- Resource Type
- Journal article