Journal article
Evaluation of a Caprine Arthritis-Encephalitis Virus/Maedi-Visna Virus Indirect Enzyme-Linked Immunosorbent Assay in the Serological Diagnosis of Ovine Progressive Pneumonia Virus in U.S. Sheep
Clinical and vaccine immunology, Vol.17(2), pp.307-310
02/2010
Handle:
https://hdl.handle.net/2376/112871
PMCID: PMC2815521
PMID: 20016044
Abstract
A caprine arthritis-encephalitis virus (CAEV)/maedi-visna virus (MVV) indirect enzyme-linked immunosorbent assay (iELISA) was validated with samples from U.S. sheep and by the use of radioimmunoprecipitation as the standard for comparison. The sensitivity and the specificity were 86.0% (±5.8%) and 95.9% (±2.9%), respectively. The iELISA format and phylogenetic differences based on the MVV
gag
sequence contribute to the reduced sensitivity.
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Details
- Title
- Evaluation of a Caprine Arthritis-Encephalitis Virus/Maedi-Visna Virus Indirect Enzyme-Linked Immunosorbent Assay in the Serological Diagnosis of Ovine Progressive Pneumonia Virus in U.S. Sheep
- Creators
- Lynn M Herrmann-Hoesing - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630Liam E Broughton-Neiswanger - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630Kimberly C Gouine - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630Stephen N White - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630Michele R Mousel - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630Gregory S Lewis - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630Katherine L Marshall - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630Donald P Knowles - U.S. Department of Agriculture, Agricultural Research Service, Animal Disease Research Unit, Washington State University, Pullman, Washington 99164-6630
- Publication Details
- Clinical and vaccine immunology, Vol.17(2), pp.307-310
- Academic Unit
- Veterinary Microbiology and Pathology, Department of; Paul G. Allen School for Global Animal Health
- Publisher
- American Society for Microbiology (ASM)
- Identifiers
- 99900548014601842
- Language
- English
- Resource Type
- Journal article