Journal article
Evaluation of bupivacaine liposome injectable suspension efficacy in single-use vials over five days of multiple use
Veterinary anaesthesia and analgesia, Vol.48(6), pp.956-961
11/01/2021
PMID: 34561182
Abstract
Objective To test the anesthetic effect of a bupivacaine liposome injectable suspension (BLIS), used in a multiple-dose manner for up to 5 consecutive days.
Study design: Prospective, randomized, experimental study. Animals A total of 30 male and female Sprague-Dawley rats (Rattus norvegicus), aged 97 (75-130) days and weighing 337.2 (219.6-465.9) g, mean (range).
Methods: Rats were assigned to one of five BLIS vial groups, in which drug was administered from a newly opened vial or 1, 2, 3 and 4 days after the vial was opened. The vials were refrigerated between uses. A 14 gauge needle attached to an injection plug was used to puncture each vial once and was not removed; BLIS was withdrawn from the injection plug in a multiple-dose fashion. A dose rate of 0.4 mL kg(-1) was administered subcutaneously into the left pelvic limb paw. Antinociception was evaluated using a paw pressure test on both injected and uninjected paws before (time 0, baseline) and 1, 24, 48 and 72 hours after injection.
Results: Age of BLIS vial had no significant effect on anesthetic efficacy (p = 0.97). Across all groups, paw withdrawal latency averaged 5.23 +/- 0.24 seconds at baseline (before BLIS injection), increased to 16.45 +/- 0.65 seconds at 1 hour after BLIS injection, declined to 7.50 +/- 0.76 seconds at 24 hours after BLIS injection, and further declined thereafter (p < 0.001). There was no significant change in paw withdrawal latency in the uninjected paw over time.
Conclusions and clinical relevance: BUS single-use vials retained efficacy when used up to 5 days in a multiple-dose fashion. Because anesthetic effects declined substantially after 24 hours, multimodal pain management remains important for providing analgesia care.
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Details
- Title
- Evaluation of bupivacaine liposome injectable suspension efficacy in single-use vials over five days of multiple use
- Creators
- Alison Y. Wang - Washington State UniversityLais Malavasi - Washington State UniversityRebecca Craft - Washington State University
- Publication Details
- Veterinary anaesthesia and analgesia, Vol.48(6), pp.956-961
- Academic Unit
- Psychology, Department of
- Publisher
- Elsevier
- Number of pages
- 6
- Grant note
- 2530-0716 / Washington State University Herbert L Eastlick Professorship
- Identifiers
- 99900907904801842
- Language
- English
- Resource Type
- Journal article