Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1

Chengtao Her; Anthony T Vo; Xiling Wu

doi:10.1016/S1568-7864(02)00079-4

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Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1

Journal article

Peer reviewed

Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1

Chengtao Her, Anthony T Vo and Xiling Wu

DNA repair, Vol.1(9), pp.719-729

09/04/2002

DOI: https://doi.org/10.1016/S1568-7864(02)00079-4

Handle:

https://hdl.handle.net/2376/104995

PMID: 12509276

Abstract

MutL Protein Homolog 1

Oligonucleotide Array Sequence Analysis

Saccharomyces cerevisiae - genetics

Humans

Open Reading Frames

Base Pair Mismatch

Gene Expression Profiling

Neoplasm Proteins - metabolism

Recombinant Fusion Proteins - metabolism

DNA-Binding Proteins - metabolism

Saccharomyces cerevisiae - metabolism

MRE11 Homologue Protein

DNA Mutational Analysis

DNA Damage - genetics

Neoplasm Proteins - genetics

Carrier Proteins

Fibroblasts - metabolism

Cell Line

DNA-Binding Proteins - genetics

Fibroblasts - pathology

Blotting, Western

Precipitin Tests

Nuclear Proteins

Two-Hybrid System Techniques

Adaptor Proteins, Signal Transducing

DNA Repair

Recombinant Fusion Proteins - genetics

In both mitotic and meiotic processes, cellular surveillance of the integrity of genetic information transmission from parental cells to their subsequent generations is carried out by a network of proteins primarily involved in cell-cycle regulation, DNA replication, DNA repair, and chromosome segregation. Within this context, the mammalian MRE11 represents an essential multifunctional protein that promotes repair of DNA double-strand breaks and plays a role in the signaling of DNA damage response. Mutations in human hMRE11 gene could contribute to the rare "AT-like" disorder. However, at present time the functional roles of hMRE11 in these cellular processes are elusive. In the current study, we provide evidence that hMRE11 interacts physically with the mismatch repair protein hMLH1 through yeast two-hybrid analysis. In addition, we show that recombinant hMRE11 and hMLH1 proteins interact when these two proteins are coexpressed in bacterial cells, and both proteins can be co-immunoprecipitated from human cell extracts. Furthermore, hMRE11 and hMLH1 display similar expression patterns when examined with a human normal/tumor DNA array. Together, these data suggest that hMRE11 and hMLH1 might act in a co-operative fashion during DNA damage detection, signaling, and repair.

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Title: Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1
Creators: Chengtao Her - School of Molecular Biosciences, Center for Reproductive Biology, P.O. Box 644660, Washington State University, Pullman, WA 99164-4660, USA. cher@wsu.edu
Anthony T Vo
Xiling Wu
Publication Details: DNA repair, Vol.1(9), pp.719-729
Academic Unit: Molecular Biosciences, School of
Publisher: Netherlands
Identifiers: 99900546567801842
Language: English
Resource Type: Journal article

Evidence for a direct association of hMRE11 with the human mismatch repair protein hMLH1

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