Journal article
Evidence for a non-antioxidant, dose-dependent role of α -lipoic acid in caspase-3 and ERK2 activation in endothelial cells
Apoptosis (London), Vol.10(3), pp.657-665
05/2005
Handle:
https://hdl.handle.net/2376/107928
PMID: 15909127
Abstract
Endothelial cell apoptosis contributes to atherosclerosis and may be exacerbated by oxidative stress. Results from clinical trials using antioxidant supplementation are equivocal and could be enhanced by antioxidants with additional non-antioxidant properties such as α -lipoic acid and α -tocopherol. The aim of this study was to investigate the effects of these antioxidants on cytoprotective pathways and endothelial apoptosis. Endothelial cells were incubated with α -lipoic acid and α -tocopherol, alone or in combination, prior to incubation with H2O2 or staurosporine. α -lipoic acid pre-treatment alone increased caspase-3 activity in a dose-dependent manner. Both H2O2 and staurosporine increased DNA fragmentation and caspase-3 activity and pre-treatment of cells with α -lipoic acid and/or α -tocopherol failed to prevent stress-induced apoptosis. Neither antioxidant treatments nor apoptotic inducers alone altered expressions of Bcl-2, Bax, HSP70 or pERK1/2 or pJNK. α -lipoic decreased pERK2 in staurosporine-treated cells in a dose-dependent manner. These findings indicate that pre-incubation with α -lipoic acid and α -tocopherol, alone or in combination, does not protect against oxidative- or non-oxidative-induced apoptosis in endothelial cells. Moreover, we have demonstrated a non-antioxidant, dose-dependent role of α -lipoic acid in caspase-3 and ERK2 activation. These data provide an insight and indicate caution in the use of high doses of α -lipoic acid as an antioxidant.
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Details
- Title
- Evidence for a non-antioxidant, dose-dependent role of α -lipoic acid in caspase-3 and ERK2 activation in endothelial cells
- Creators
- S Marsh - School of Human Movement Studies The University of Queensland Brisbane AustraliaB Pat - School of Human Movement Studies The University of Queensland Brisbane AustraliaG Gobe - School of Human Movement Studies The University of Queensland Brisbane AustraliaJ Coombes - School of Human Movement Studies The University of Queensland Brisbane Q 4072 Australia
- Publication Details
- Apoptosis (London), Vol.10(3), pp.657-665
- Academic Unit
- Pharmaceutical Sciences, Department of
- Publisher
- Kluwer Academic Publishers; Boston
- Identifiers
- 99900547191801842
- Language
- English
- Resource Type
- Journal article